MCT1在不同发育时期及缺血缺氧脑白质损伤大鼠胼胝体内的表达  

Expression of MCT1 in corpus callusom of rat in different development periods and following hypoxia-ischemia induced brain white matter damage

作　　者：江红艳[1] 刘守正[1] 张智慧[1] 程冉[1] 张振中[2] 余浩[2] 姚瑞芹[2] 

机构地区：[1]沭阳县人民医院康复科,沭阳223600 [2]徐州医学院细胞生物学及神经生物学教研室,徐州221009

出　　处：《神经解剖学杂志》2016年第2期179-184,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81271345);江苏省自然科学基金(BK20131132);江苏省2014高校年度"青蓝工程"中青年学术带头人资助项目(姚瑞芹)

摘　　要：目的:观察单羧酸转运体-1(monocarboxylate transporter-1,MCT1)在不同发育时期及缺血缺氧(hypoxia-ischemia,HI)损伤模型大鼠胼胝体内的表达。方法:采用多聚甲醛灌注固定7 d、14 d、21 d和28 d SD大鼠脑组织,冰冻切片后进行MCT1/CNPase和MCT1/GFAP免疫荧光双标,观察胼胝体内MCT1在少突胶质细胞和星形胶质细胞的表达;另选生后3 d的SD大鼠,右侧颈总动脉结扎及缺氧处理以建立HI性脑白质损伤模型,至生后28d观察胼胝体MCT1的表达。结果:MCT1分别与CNPase和GFAP免疫荧光双标显示,生后早期,正常大鼠胼胝体内MCT1主要在GFAP阳性细胞表达,随生长时间延长,MCT1在CNPase阳性细胞的表达增加,而在GFAP阳性细胞的表达逐渐减少;HI损伤后28 d,MCT1/GAFP免疫荧光强度较对照组显著升高(P<0.01),而MCT1/CNPase的表达较对照组显著降低(P<0.01)。结论:在成年SD大鼠胼胝体,MCT1主要在CNPase阳性的少突胶质细胞表达,而HI脑白质损伤后,MCT1主要在星形胶质细胞表达。Objective： To observe the changes of monocarboxylate transporter-1（MCT1） expression in corpus callusom of rat in different developmental stages and following hypoxia-ischemia（ HI） induced brain white matter damage（WMD）. Methods： Sprague-Dawley rats were perfused by paraformaldehyde at postnatal 7 d、14 d、21 d and 28 d.MCT1 / CNPase and MCT1 / GFAP immunofluorescence double labeling was performed on cryosections. Three day-old Sprague-Dawley rats were subjected to right common carotid artery ligation followed by 2 hours of exposure to 8% O2 to induce WMD. Immunofluorescence staining was used to observe the expression of MCT1 in WMD corpus callusom till postnatal 28 d. Results： Double labeling of MCT1 and CNPase or GFAP showed that MCT1 was mainly expressed in GFAP positive astrocytes at early postnatal. The expression of MCT1 was increased in CNPase positive oligodendrocytes and decreased in GFAP positive astrocytes with the development in normal rats. However,the expression of MCT1 / GAFP was significantly enhanced（ P〈 0. 01） and MCT1 / CNPase was decreased（ P〈 0. 01） in postnatal 28 d WMD rat corpus callusom. Conclusion： MCT1 is dominantly expressed in CNPase＋oligodendrocytes in the normal adult SD rat corpus callusom,while it is mainly expressed in GFAP＋astrocytes following HI induced brain WMD.

关 键 词：单羧酸转运体-1 胼胝体 发育 少突胶质细胞 缺血缺氧 

分 类 号：R741[医药卫生—神经病学与精神病学]