FOXA2在人胃癌中的表达及预后意义  

Expression of FOXA2 in gastric carcinoma and its prognostic significance

作　　者：彭俊文[1] 陈健[2] 许海林[1] 李建田[1] 许朝阳[3] 

机构地区：[1]建德市第一人民医院普外科,浙江建德311600 [2]浙江大学医学院附属第二医院普外科,浙江杭州310009 [3]建德市第一人民医院病理科,浙江建德311600

出　　处：《实用肿瘤杂志》2016年第2期150-153,共4页Journal of Practical Oncology

摘　　要：目的研究叉头框转录因子A2(forkhead box A2,FOXA2)在人胃癌组织中的表达水平及其对患者预后的影响。方法收集行手术切除的胃癌及对应癌旁胃组织57例。运用反转录-实时定量PCR(quantitative reverse transcription-PCR,q RT-PCR)技术检测在胃癌及对应癌旁组织中FOXA2 m RNA的表达水平,免疫组织化学法检测组织标本中FOXA2蛋白的表达情况,分析FOXA2蛋白表达与患者临床特征间的相关性,采用Kaplan-Meier生存曲线分析FOXA2蛋白表达水平对患者预后的影响。结果胃癌组织中FOXA2 m RNA和蛋白的阳性表达水平均低于对应癌旁组织(P<0.05)。胃癌组织中FOXA2蛋白阴性表达在淋巴结转移(P<0.05)及高TNM分期(Ⅲ+Ⅳ期,P<0.05)患者中较多。与FOXA2蛋白表达阳性患者(16例)比较,FOXA2蛋白阴性表达的患者(41例)3年总生存率及无瘤生存率均较低(均P<0.05)。结论 FOXA2在胃癌组织中表达下调并与胃癌恶性临床病理特征及不良预后有关。Objective To investigate the expression of forkhead box A2（ FOXA2） in gastric carcinoma and its prognostic significance. Methods Fifty-seven specimens of gastric carcinoma and matched tumor-adjacent tissue were collected. The mRNA level of FOXA2 was detected by quantitative reverse transcription-PCR（ qRT-PCR）,and the protein level of FOXA2 was detected by immunohistochemistry. The correlation between FOXA2 expression and clinicopathological features was analyzed. The prognosis of patients was analyzed by Kaplan-Meier survival curve. Results Positive expression rates of FOXA2 mRNA and protein were lower in gastric carcinoma tissue,compared with matched tumor-adjacent tissue（ P〈0. 05）. Negative expression of FOXA2 was more common in patients with lymphatic metastasis（ P〈0. 05） and advanced TNM stage（ Ⅲ ＋ Ⅳ,P〈0. 05）. Compared with FOXA2-positive gastric cancer patients（ n = 16）,the 3-year overall survival rate and disease-free survival rate were both lower in FOXA2-negative patients（ n = 41,P〈0. 05）. Conclusion Downregulation of FOXA2 in human gastric carcinoma is related to malignant clinicopathological features and poor prognosis.

关 键 词：胃肿瘤/病理学 叉头转录因子类 基因表达 逆转录聚合酶链反应 免疫组织化学 预后 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]