IL-23A和IL-17A在CIN及子宫颈癌中的表达及意义  

Expressions of IL-23A and IL-17A in CIN and SCC and their clinical significance

作　　者：高俊[1] 李艳琴[1] 刘兮[1] 陈燕[1] 高鑫[1] 

机构地区：[1]成都双流县妇幼保健院妇产科,四川双流610200

出　　处：《实用肿瘤杂志》2016年第2期157-162,共6页Journal of Practical Oncology

摘　　要：目的探讨白细胞介素23A（interleukin-23A,IL-23A）和IL-17A在不同等级子宫颈上皮内瘤变（cervical intraepithelial neoplasia,CIN）及子宫颈鳞状细胞癌中的表达水平及相关性。方法收集临床标本89例,其中20例健康对照（无感染或病变）,11例低级别CIN,36例高级别CIN（CINⅡ-Ⅲ）;22例子宫颈鳞状细胞癌。ELISA检测血清及子宫颈分泌物中IL-23A和IL-17A的表达及其相关性。第二代杂交捕获试验检测子宫颈分泌物中人乳头状瘤病毒（human papillomavirus,HPV）DNA的表达情况,分析IL-23A和IL-17A的表达与临床预后及HPV感染的关系。结果与对照组比较,IL-23A和IL-17A在CIN和子宫颈癌分泌物及血清中呈高表达,差异有统计学意义（均P〈0.05）。子宫颈癌患者血清IL-23A与IL-17A的表达呈正相关（r=0.865,P〈0.01）,子宫颈癌患者血清IL-23A、IL-17A高表达患者的生存时间明显缩短（均P〈0.01）。IL-23A和IL-17A的表达与HPV的感染有关（P〈0.01）。结论 IL-23A和IL-17A在子宫颈上皮内瘤变和子宫颈癌中高表达,有望作为早期预测子宫颈病变的生物标志物。Objective To investigate the expressions of IL-23 A and IL-17 A in cervical intraepithelial neoplasia（CIN） and squamous cervical carcinoma（SCC） and their clinical significance. Methods Twenty-two patients with squamous cervical carcinoma（ SCC）,47 patients with cervical intraepithelial neoplasia（ including 11 with CIN I and 36 with CIN Ⅱ-Ⅲ） and 20 healthy women（ control group） were enrolled in this study. The IL-23 A and IL-17 A levels in the cervical secretions and serum were examined by enzyme-linked immunosorbent assay（ ELISA）. Human papillomavirus（ HPV）DNA in cervical smears was detected by PCR screening kits. The correlations of IL-23 A and IL-17 A levels with cervical disorders and with HPV infection were analyzed. Results Compared with the control group,IL-23 A and IL-17 A levels were both increased in cervical secretions and in serum of patients with CIN and SCC（ P〈0. 05）. The levels of IL-23 A and IL-17 A in the serum of SCC patients were positively correlated（ r = 0. 865,P〈0. 01）. The IL-23 A and IL-17 A levels were negatively associated with the survival of SCC patients（ P〈0. 01）. IL-23 A and IL-17 A levels were associated with HPV infections（ P〈0. 01）. Conclusion Elevated levels of IL-23 A and IL-17 A in cervical secretion and serum are observed in CIN and SCC patients,which may serve as additional biomarkers for diagnosis and prognosis of cervical disorders.

关 键 词：宫颈肿瘤/病理学 宫颈上皮内瘤样病变/病理学 肿瘤 鳞状细胞 白细胞介素17 白细胞介素23 基因表达 酶联免疫吸附测定 预后 

分 类 号：R737.33[医药卫生—肿瘤] R730.23[医药卫生—临床医学]