福建部分地区人和动物嗜吞噬细胞无形体基因检测和序列分析  

Genetic identification and sequene analysis for high-risk group and animals of Anaplasma phagocytophilum infection in some areas of Fujian Province,China

作　　者：肖方震[1] 李丹萍[2] 徐国英[1] 陈阳[1] 邓艳琴[1] 

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州350001 [2]福建医科大学,福州350004

出　　处：《中国人兽共患病学报》2016年第3期262-265,共4页Chinese Journal of Zoonoses

基　　金：福建省医学创新课题(No.2012-CXB-12)资助~~

摘　　要：目的了解福建省人和动物嗜吞噬细胞无形体的感染状况以及基因特征。方法收集永春县和上杭县高危人群、家畜的全血,采用巢式PCR扩增无形体16SrRNA片段,PCR产物纯化测序并与序列比对分析。结果永春县牛的无形体感染率最高为53.85%,羊、狗无形体感染率分别为24.71%和12.50%。上杭县羊的无形体感染率为53.06%,高危人群的无形体感染率为8.33%。无形体基因序列与相应的参考序列同源性均达97%~100%。结论福建省人和动物存在嗜吞噬细胞无形体感染。To explore the nature infection and gene characteristics of Anaplasma phagocytophilum（A.phagocytophilum）in high-risk groups and animals of Fujian Province,China,blood samples from high-risk groups and animals were collected in Yongchun and Shanghang regions,for which nest polymerase chain reaction（PCR）was used to amplify the fragments of 16 s rRNA gene of A.phagocytophilum.In addition,the positive PCR products were sequenced and compared with the corresponding sequences acquired by biological analysis software.The infection rate of ox,goats and dogs in Yongchun region were determined to be 53.85%,24.71% and 12.50%,and the infection rates of goats and high-risk groups in Shanghang region were 53.06% and 8.33%,respectively.The nucleotide sequence homologies of partial 16 srRNA gene for both the objective and reference sequences concurrently reached up to 97%-100%.It was concluded that A.phagocytophilum was found in some regions of Fujian Province and it should be paid more attention in the following years.

关 键 词：无形体 巢式PCR 16S RRNA 序列分析 

分 类 号：R372[医药卫生—病原生物学]