Ro60表达下调抑制肺腺癌A549细胞迁移和侵袭  

Downregulation of Ro60 inhibits migration and invasion of human lung adenocarcinoma A549 cells

作　　者：姚婉[1] 刘继来[1] 孙鹏达[2] 田宝磊[1] 刘斌[1] 宋宜[1] 孙志贤[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,放射生物学北京市重点实验室,北京100850 [2]吉林大学第二医院,长春130000

出　　处：《军事医学》2016年第3期213-216,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81372252,31201014)

摘　　要：目的探究Ro60对肺腺癌A549细胞迁移和侵袭的影响。方法采用RNA干扰(RNAi)方法下调A549细胞中Ro60表达水平,检测其迁移和侵袭能力的改变,通过实时定量PCR和免疫印迹方法分析与肿瘤侵袭转移相关分子的表达变化。结果下调Ro60表达,A549细胞的迁移和侵袭能力均减弱,与肿瘤细胞迁移和侵袭相关的MMP9、c-Src等在mRNA水平明显降低,Src蛋白和MMP9酶原激活形式蛋白表达量下调。结论 Ro60表达下调抑制A549细胞的迁移和侵袭,并通过调控Src蛋白表达和MMP9酶原激活发挥作用。Objective To study the effect of Ro60 on migration and invasion of lung adenocarcinoma A549 cells.Methods We knocked down Ro60 and analyzed the ability of migration and invasion of A549 cells. Quantitative real time（ RT）-PCR and Western blotting were performed to detect mRNA and protein levels of selected molecules associated with migration and invasion of neoplasm. Results When Ro60 was downregulated,the migration and invasion of A549 cells decreased markedly. Meanwhile,the mRNA expression of matrix metalloproteinase（ MMP） 9,c-Src etc was observably reduced. Furthermore,downregulation of Ro60 diminished the expression of Src protein and the activation of MMP-9protein. Conclusion Downregulation of Ro60 inhibits migration and invasion of A549 cells by regulating Src protein and activating MMP-9 protein.

关 键 词：Ro60 肺腺癌细胞 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]