EPL和PTS在生物合成主链环肽方面的应用研究进展  

Research progress in biosynthesis of backbone-cyclized peptides using EPL and PTS

作　　者：叶丙雨[1] 赵志虎[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《军事医学》2016年第3期252-255,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31030026;31370762)

摘　　要：内含肽作为宿主蛋白的组成成分,不仅可将自身从宿主蛋白中剪切,而且还可催化外显肽的连接。内含肽广泛分布于生物界的三大领域及病毒蛋白中。蛋白质剪接是由内含肽介导的一种翻译后自催化加工过程,整个过程不需要酶或辅因子的参与。蛋白质剪接包括四步分子内的反应及内含肽和外显肽中部分发挥关键催化功能的氨基酸残基。表达蛋白质连接(EPL)和蛋白质反式剪接(PTS)技术是借助内含肽及蛋白质剪接发展起来的。该文主要就这两项技术在生物合成主链环肽文库方面的应用领域及未来的应用前景进行了综述。As internal protein elements,inteins are self-excised from their host protein and catalyze ligation of exteins with a peptide bond. They are widely found in all the three domains of the biological kingdom,and in viral proteins. Inteinmediated protein splicing is a posttranslational autocatalytic process that does not require auxiliary enzymes or cofactors.Protein splicing involves four intramolecular reactions and a small number of key catalytic residues in the intein and exteins.The development of expressed protein ligation（ EPL） and protein trans-splicing（ PTS） is assisted by inteins and protein splicing. This review introduces the biosynthesis of backbone-cyclized peptides libraries,and describes the applications of cyclic polypeptides and their prospective applications in the future.

关 键 词：蛋白质剪接 EPL PTS 肽生物合成 

分 类 号：Q51[生物学—生物化学]