连作条件下谷子叶片衰老与活性氧代谢研究  

Leaf senescence and reactive oxygen metabolism of millet under continuous cropping

作　　者：李夏[1] 妙佳源 高小丽[1] 杨璞[1] 高扬[1] 冯佰利[1] 

机构地区：[1]西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《中国农业大学学报》2016年第4期1-9,共9页Journal of China Agricultural University

基　　金：国家谷子糜子产业技术体系(CARS-07-12.5-A9);陕西省科技统筹创新工程计划项目(2014KTZB02-03);陕西省科学技术研究发展计划项目(2014K01-28);陕西省小杂粮产业技术体系(2015)

摘　　要：为研究不同连作年限下谷子功能叶片衰老和活性氧代谢特性,加深对谷子连作障碍的认识,以晋谷29号品种为材料,选取3个不同年限(2、3和4年)的连作方式,以轮作方式为对照,分析不同连作年限下,功能叶片叶绿素、可溶性蛋白含量、保护酶活性(SOD、POD、CAT)、膜脂过氧化产物丙二醛(MDA)和氧自由基积累量等生理指标的变化。对比轮作与连作处理,结果表明:谷子开花后,其功能叶片的叶绿素、可溶性蛋白含量、CAT、SOD和POD活性均呈下降趋势,MDA和氧自由基积累量持续上升。其中:叶绿素和可溶性蛋白含量随着连作年限的增加显著下降(P<0.05);CAT活性随着连作年限的增加而下降;SOD和POD活性在开花前中期随连作年限的增加呈显著降低趋势;MDA和氧自由基积累量上升,且连作高于轮作。相关性分析显示,花后功能期叶片SOD活性与产量呈正相关,POD活性、叶绿素含量、CAT活性和可溶性蛋白与产量呈显著或极显著正相关,氧自由基、MDA含量与产量呈显著负相关。连作影响谷子功能叶片代谢,增加连作年限,叶片功能期缩短,植株衰老加剧,最终导致谷子产量降低。To explore the impact of continuous cropping on millet growth,development and yield and investigate the physiological mechanisms of continuous cropping,Jingu 29 was selected as test material with 4rotations：kidney bean crop rotation（CK）and different continuous cropping years（2-4years）.Seven biological indexes（chlorophyll content,soluble protein content,SOD activity,POD activity,CAT activity,MDA content and ORF content）were measured.The results showed：After flowering,the contents of chlorophyll and soluble protein and the activity of CAT,SOD and POD showed downtrend,while the accumulation of MDA and OFR kept elevating;With the increase of the continuous cropping years,the contents of chlorophyll and soluble protein decreased significantly（P〈0.05）,the activity of CAT decreased,the activity of SOD and POD decreased significantly on prometaphase of anthesis,the accumulation of MDA and OFR increased,and the contents of continuous cropping are higher than rotation cropping.The correlation analysis showed that the grain yield had a positive correlation with the activity of SOD,and had a significant or very significant positive correlation with the activity of POD and CAT,the content of chlorophyll and soluble protein,while had a significant negative correlation with the accumulation of MDA and OFR.With the increased of millet continuous years,the functional period of leaf shortened,the degree of reactive oxygen damage aggravated,and the grain field decreased.

关 键 词：谷子 连作 轮作 活性氧代谢 产量 叶片衰老 

分 类 号：S515.01[农业科学—作物学]