甲硫氨酸γ-裂解酶效价测定方法的研究  

Study on Titer Measurement of Methionine γ-Lyase

作　　者：谢鹏 梁淑娃 秦鹏 胡海艳 

机构地区：[1]广州市微生物研究所,广东广州510663

出　　处：《今日药学》2016年第3期162-164,171,共4页Pharmacy Today

基　　金：广州市科技型中小企业技术创新基金项目(201601010033)

摘　　要：目的探讨测定甲硫氨酸γ-裂解酶效价的方法。方法用紫外分光光度法,以α-丁酮酸为对照品,测定波长为315 nm,测定甲硫氨酸γ-裂解酶效价。结果甲硫氨酸γ-裂解酶效价测定的回归线性方程为:y=0.003 2x+0.009 1,相关系数R2=0.999 1。平均回收率为99.85%,RDS为0.36%。结论本法准确、稳定、可靠,适合作为甲硫氨酸γ-裂解酶效价测定。OBJECTIVE To study the titer measurement method of Methionine γ-lyase（ MGL）. METHODS UV spectrophotometry was used,with α-Ketobutyric acid as the reference substance,and the detection wavelength was set at 315 nm.RESULTS As a result,the regression equation was： y = 0. 003 2x ＋ 0. 009 1,and the correlation coefficient（ R2） was 0. 999 1.Average recovery ratio for MGL was 99.85%,with a RSD of 0.36%. CONCLUSION Accordingly the method is accurate,stable and reliable so that it may be applied to determinate the titer of MGL.

关 键 词：甲硫氨酸γ-裂解酶 效价测定 α-丁酮酸 紫外分光光度法 

分 类 号：R962[医药卫生—药理学]