重金属对浒苔吸收硝酸盐的动力学特性影响  

Effects of heavy metals on the uptake of nitrate by Ulva prolifera

作　　者：吴老五 韩秀荣[1,2] 吴婷[3] 杜锦[1,2] 石晓勇[1,2,3] 

机构地区：[1]中国海洋大学化学化工学院,山东青岛266100 [2]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100 [3]国家海洋局海洋减灾中心,北京100194

出　　处：《中国环境科学》2016年第4期1173-1180,共8页China Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2010CB428701)

摘　　要：为探讨大型海藻对重金属胁迫的响应,选取浒苔(Ulva prolifera)为实验材料,通过实验室一次性培养实验,从大型藻营养盐吸收动力学角度,研究了Cu(II)、Zn(II)和Pb(II)对浒苔吸收硝酸盐动力学参数的影响.结果表明:较低浓度的Cu(II)(<0.04mg/L)和Zn(II)(<0.12mg/L)对浒苔吸收硝酸盐具有促进作用;当达到一定浓度(Cu(II)>0.10mg/L和Zn(II)>0.20mg/L)时则对浒苔吸收硝酸盐表现为明显的抑制作用,Pb(II)对浒苔吸收硝酸盐均是抑制作用.Cu(II)、Zn(II)和Pb(II)对浒苔吸收硝酸盐的最大吸收速率(Vmax)抑制率IPC50分别为0.23,0.66,0.63mg/L.当添加重金属浓度大于0.50mg/L时,重金属对浒苔吸收硝酸盐的抑制顺序为:Cu(II)最强,Pb(II)次之,Zn(II)最小.In order to investigate the response of the macroalgae to heavy metal contamination, the influence of three heavy metal ions（Cu（II）, Zn（II） and Pb（II）） on the uptake of nitrate by Ulva prolifera was investigated. Kinetic parameters related to nitrate uptake in different trials were determined by the batch culture experiment in the laboratory. The results showed that the uptake of nitrate by Ulva prolifera was promoted at low concentrations of Cu（II）（0.04mg/L） and Zn（II）（0.12mg/L） and inhibited at high concentrations（Cu（II）0.10mg/L and Zn（II）0.20mg/L）. Pb（II） played a negative role in the uptake of nitrate by Ulva prolifera at all tested concentrations. The inhibition rate of the maximum absorption rate of nitrate（Vmax） were calculated to be 0.23mg/L, 0.66mg/L, and 0.63mg/L for Cu（II）, Zn（II）, and Pb（II）, respectively. The inhibition effects of the three heavy metals at concentrations 0.5mg/L on Ulva prolifera nitrate uptake were observed to be in the order of Cu（II）Pb（II）Zn（II）.

关 键 词：浒苔 硝酸盐 重金属 

分 类 号：X171.5[环境科学与工程—环境科学]