苯醚甲环唑对斑马鱼抗氧化酶的影响  

Impact of difenoconazole on the antioxidases of zebrafish(Danio rerio)

作　　者：穆希岩 黄瑛[2] 沈公铭[2] 柴婷婷[1] 朱丽珍[1] 李绪兴[2] 雷云雷[2] 冯庚菲[2] 李应仁[2] 李学锋[1] 王成菊[1] 

机构地区：[1]中国农业大学理学院,北京100193 [2]中国水产科学研究院资源与环境研究中心,北京100141

出　　处：《中国环境科学》2016年第4期1242-1249,共8页China Environmental Science

基　　金：农业部科研专项;农药风险评估综合配套技术研究-水生生物慢性毒性方法(200903054-02);农业部财政项目物种资源保护(2130135)

摘　　要：以斑马鱼为模式生物研究了三唑类杀菌剂苯醚甲环唑对斑马鱼脑和肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽还原酶(GR)四种抗氧化酶活性的影响.结果发现:苯醚甲环唑暴露后,斑马鱼脑中CAT酶活性随暴露时间延长呈现出先升高后降低的趋势.50μg/L及更高浓度的苯醚甲环唑能够显著抑制斑马鱼脑和肝脏中GPx的活性,且对脑中GPx活性的抑制程度更强.另外,500μg/L苯醚甲环唑处理后,斑马鱼肝脏和脑中GR活性表现出不同的变化趋势,肝脏中GR活性下降,而脑中GR活性升高.上述结果表明50μg/L的苯醚甲环唑即可影响斑马鱼的抗氧化系统,其对农业水域中的鱼类影响值得重视.To investigate the effect of difenoconazole on zebrafish antioxidases, adult zebrafish were exposed to three concentrations of difenoconazole for 15 days. The activity of superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase（GPx） and glutathione reductase（GR） was measured at 3, 8 and 15 days post exposure（dpe） respectively. Results indicated that the brain CAT activity was increased at 3 and 8 dpe but decreased at 15 dpe. 50μg/L or higher difenoconazole could inhibit brain and hepatic GPx activity of zebrafish, and the inhibition in brain was stronger than liver. In addition, brain GR activity was induced by 500μg/L difenoconazole while hepatic GR activity was reduced under the same exposure. These results implicated that 50μg/L could alter the antioxidant system of zebrafish and its effects on fish living in agricultural water areas should be concerned.

关 键 词：苯醚甲环唑 斑马鱼 抗氧化酶 脑 肝脏 

分 类 号：X171.5[环境科学与工程—环境科学]