重度抑郁障碍与肿瘤坏死因子ɑ和白细胞介素6基因启动子区多态性的相关性  

Association of polymorphisms in promoter region of tumor necrosis factor and interleukin 6 gene with major depressive disorder

作　　者：马心慧[1] 付瑜[1] 曹淑杰 王双力[1] 武伟晶[1] 王军利[1] 王芳[1] 

机构地区：[1]武警黑龙江总队医院检验科,哈尔滨150076 [2]黑龙江省哈尔滨市第一专科医院检验科,150001

出　　处：《武警医学》2016年第3期259-262,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨肿瘤坏死因子α（tumor necrosis factor,TNF-α）基因启动子区-308G/A、-857C/T位点,白细胞介素6（interleukin-6,IL-6）基因启动子-174G/C和-572C/G多态性与重度抑郁障碍症发生的相关性。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析方法检测393例重度抑郁障碍症患者和393名健康者对照各个多态性位点的基因型,采用SPSS 13.0进行统计学分析。结果 TNF-α基因启动子-857C/T位点的基因型及等位基因频率分布在重度抑郁障碍症组与正常对照组间存在统计学意义（P〈0.05）。其中,-857T的等位基因频率只在男性重度抑郁障碍症组与对照组间存在显著差异（OR=0.309,95%CI：0.218~0.439,P〈0.01）,而-308A位点的等位基因频率只在女性病例组与对照组间存在统计学意义差异（OR=0.399,95%CI：0.246~0.648,P〈0.01）。结论证实TNF-α基因启动子区多态性可能是中国北方汉族重度抑郁障碍患者的风险因素。TNF-α基因启动子区多态性可能与重度抑郁障碍的发病存在关联。Objective To investigate the relationship between the-308G/A and-857C/T of tumor necrosis factorαgene（ TNF-α）,-174 G /C and-572 C /G of interleukin-6 gene（ IL-6） polymorphisms and major depressive disorder. Methods Genomic DNA was isolated from the venous blood leukocytes of 393 unrelated patients with major depressive disorder and 393 healthy unrelated individuals（ control group）. All of the polymorphisms were genotyped by PCR-restriction fragment length polymorphisms（ PCR-RFLP）. Genotype and allele frequencies were analyzed using SPSS 13. 0 software. Results There were significant differences in both allele and genotype frequencies of-857 C / T of TNF-αgene between the major depressive disorder and control groups（ P〈0. 05）. The allele T of-857 T in male major depressive disorder group was significantly higher than that in contol group（ OR = 0. 309,95% CI： 0. 218- 0. 439,P〈0. 01） The allele A of-308 A in female major depressive disorder group was significantly higher than that in contol group（ OR =0. 399,95% CI： 0. 246- 0. 648,P〈0. 01）. Conclusions This study confirm that TNF-α gene promoter region gene polymorphism may be risk factors for severe depressive disorder in North China Han. TNF-α gene promoter polymorphism may be associated with the onset of severe depressive disorder.

关 键 词：重度抑郁障碍症 肿瘤坏死因子Α 白细胞介素6 遗传多态性 

分 类 号：R749.3[医药卫生—神经病学与精神病学]