亚低温对脑缺血再灌注损伤的治疗作用及其机制的初探  

The effect of mild hypothermia on the treatment of cerebral ischemia reperfusion injury and the exploration of its mechanism

作　　者：贾玉龙[1] 李力仙[1] 刘龙[1] 赵锴[1] 韩鹏[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经外科,150001

出　　处：《国际免疫学杂志》2016年第2期111-115,共5页International Journal of Immunology

基　　金：国家自然科学基金（81371301）

摘　　要：目的观察亚低温对脑缺血再灌注损伤的治疗效果，初步探讨其治疗机制。方法本实验利用物理降温法，将术中大鼠体温维持在（32．0—33．0）℃，于缺血再灌注后选取5个时间点（6h、12h、24h、48h和72h）取材进行组织及基因学检测。结果亚低温有降低脑缺血再灌注术后大鼠死亡率，同时亚低温对于缺血再灌注后的脑组织具有保护作用，基因芯片筛选出亚低温组与常温组表达量变化幅度较大的基因及通路。结论亚低温能够降低脑缺血再灌注后的损伤，并通过免疫相关反应进行调节。Objective Discuss the effect of mild hypothermia on the treatment of cerebral isch^mia reperfusion injury and preliminarily study its mechanism. Methods In this article, we use physical cooling method to maintain the temperature at 32 ~C, choose five time points （6,12,24,48 and 72 h） to kill the rats af- ter reperfusion and test in histology. Result We found mild hypothermia reduced the rat mortality rate and neu- runctional score also showed mild hypothermia protected cerebral tissue after injury. Gene chip screening hypo- thermia group and normal group expression quantity change in the larger genes and pathways. Conclusions The mild hypothermia can reduce cerebral ischemia reperfusion injury, and through the immune response.

关 键 词：脑缺血再灌注 亚低温 基因芯片 

分 类 号：R743[医药卫生—神经病学与精神病学]