树鼩消化道胰高血糖素样肽-1阳性细胞分布的研究  

Research on distribution of gastrointestinal peptide glucagon-1 of positive cells in tree shrews

作　　者：陈丽雄[1] 王俊斌[1] 刘汝文[2] 吕龙宝[3] 陈丽玲[2] 

机构地区：[1]中国医学科学院医学生物学研究所,云南昆明650118 [2]昆明医科大学实验动物学部,云南昆明650500 [3]中国科学院昆明动物研究所动物实验中心,云南昆明650223

出　　处：《局解手术学杂志》2016年第4期255-257,共3页Journal of Regional Anatomy and Operative Surgery

基　　金：云南省应用基础研究面上基金(2011FZ107)

摘　　要：目的观察树鼩胃肠道胰高血糖素样肽-1(GLP-1)免疫反应阳性细胞(EG细胞)分布情况与大鼠消化道的差异,以期为树鼩模型的进一步使用提供参考。方法免疫组化SABC法检测树鼩消化道(口腔、咽除外)GLP-1的细胞阳性含量。结果EG细胞分布于树鼩十二指肠、回肠、结肠、空场中的腺上皮细胞间,阳性物质分布于细胞质。树鼩胃、直肠未见EG细胞分布。回肠免疫组织化学染色结果显示EG细胞阳性,消化道中只有回肠、结肠分布GLP-1阳性较多,树鼩与大鼠相比较消化道中GLP-1差异显著(P<0.01)。结论树鼩胃肠道内十二指肠、回肠和结肠、空肠都有胰高血糖素阳性细胞。大鼠只在回肠、结肠有阳性细胞。据GLP-1的作用机理和树鼩消化道GLP-1的分布,树鼩动脉粥样硬化(atherosclerosis,AS)AS模型不易制作可能与GLP-1的作用相关。Objective To observe tree shrews gastrointestinal glucagon like peptide 1（ glp-1） immune response positive cells（ EG cells） distribution and the difference of the digestive tract of rats. Methods Immunohisto chemical SABC method to detect tree shrews digestive tract（ except for oral cavity,pharynx） glp-1 cells positive content. Results EG cells distributed in tree shrews duodenum,ileum and colon,glandular epithelial cells of jejunum,positive material is distributed in the cytoplasm. Tree shrews stomach,rectum did not see EG cell distribution. Ileal immune dyeing results show positive,EG cells of the digestive tract,only the ileum and colon distribution glp-1 more positive,tree shrews compared with the rats in the digestive tract glp-1 significant difference（ P〈0. 01）. Conclusion Gastrointestinal tree shrew in duodenum,ileum and colon,jejunum have glucagon positive cells. The rat only have positive cells in the ileum and colon. According to the mechanism of the glp-1 and the distribution of tree shrews gastrointestinal glp-1,tree shrews atherosclerosis（ atherosclerosis,AS） AS the model is not easy to make may be associated with the role of glp-1.

关 键 词：胰高血糖素样肽-1 消化道 树鼩 模型 

分 类 号：R574.4[医药卫生—消化系统] Q578[医药卫生—内科学]