基于DNA银铂双金属纳米簇的电化学传感器用于Hg^(2+)的检测  

Electrochemical Sensor for Detection of Mercury(Ⅱ) Based on DNA-templated Ag/Pt Bimetallic Nanoclusters

作　　者：傅贤明[1] 刘智晶 蔡淑贤[2] 李萍[1] 李云腾[1] 陈敬华[2] 

机构地区：[1]莆田学院药学与医学技术学院,福建莆田351100 [2]福建医科大学药学院药物分析学系,福建福州350108

出　　处：《分析测试学报》2016年第4期426-431,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21375017;21105012);福建省自然科学基金杰出青年人才项目(2013J06003);福建省科技厅重点项目(2013Y0045);福建省高等学校新世纪优秀人才支持计划资助(JAl3130);福建省卫生系统中青年骨干人才培养项目(2014-ZQN-ZD-26);福建省大学生创新创业训练计划项目(莆田学院201511498053)

摘　　要：以DNA为模板,通过一步法合成了一种银铂双金属纳米簇(DNA-Ag/Pt NCs),其粒径为2~4 nm,并表现出较强的过氧化物模拟酶活性,能催化H2O2氧化TMB使溶液变蓝色。基于此特性,结合Hg2+可与胸腺嘧啶碱基形成T-Hg2+-T结构,设计研制了一种非标记的电化学传感器,用于Hg2+的灵敏特异性检测。实验结果表明,在最佳条件下,该传感器的检测范围为0.65~3.5 nmol/L,检出限达0.17 nmol/L,能较好地识别Hg2+。该传感器有望用于实际水样中痕量汞的检测。In this paper,a facile one-step approach to synthesize DNA-templated Ag / Pt bimetallic nanoclusters was developed.The obtained DNA-templated Ag / Pt bimetallic nanoclusters（DNA-Ag / Pt NCs） with diameters about 2-4 nm display a high efficient peroxidase-like catalytic activity,which can catalyze hydrogen peroxide（H2O2）-mediated oxidation of the substrate,3,3＇,5,5＇-tetramethylbenzidine（TMB）,to produce a blue color reaction.Based on this,the hairpin structure of thymine-Hg^2 ＋-thymine（T-Hg^2 ＋-T） interaction was utilized to design an unlabeled DNA electrochemical sensor for the Hg^2 ＋detection with high sensitivity and selectivity.Under the optimum conditions,the sensor showed a good linear relationship in the range of 0.65-3.5 nmol / L with a detection limit as low as 0.17 nmol / L and also exhibited an ultra high discrimination ability for Hg^2 ＋.All above properties make the sensor a promising candidate for Hg2 ＋trace detection in real water samples.

关 键 词：DNA模板 双金属纳米簇 模拟酶 汞离子 电化学传感器 

分 类 号：O657.1[理学—分析化学] Q524[理学—化学]