越鞠丸醇提物对孕前应激诱导的产后抑郁小鼠模型的快速抗抑郁作用  

Rapid Antidepressant Effect of Yueju Ethanol Extract on Postpartum Depression Model Mice Induced by Pre-Pregnancy Stress

作　　者：夏宝妹[1] 张海楼[1] 薛文达[1] 陈畅[1,2] 吴如燕 任荔[1] 陶伟伟[1] 陈刚[1] 

机构地区：[1]南京中医药大学基础医学院中医脑病重点实验室,江苏南京210023 [2]南京中医药大学第一临床医学院,江苏南京210023

出　　处：《武汉大学学报（医学版）》2016年第3期350-353,394,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81403041);江苏省高校优势学科支持项目(PAPD)

摘　　要：目的:以孕前应激诱导建立产后抑郁(PPD)小鼠模型,观察越鞠丸醇提物(YJ)对该模型的快速抗抑郁作用。方法:将40只BALB/c雌鼠随机分为2组,空白对照组不予任何刺激,孕前应激组给予慢性束缚刺激。3周后孕前应激组小鼠雌雄合笼交配怀孕,于分娩后3周检测模型组母鼠行为学,包括蔗糖偏好测试(SPT)、强迫游泳测试(FST)、悬尾测试(TST)及陌生环境摄食实验(NSFT)。造模成功后,将模型组随机分为4组:模型组、越鞠丸醇提物组、氯胺酮组、氟西汀组。越鞠丸醇提物及氯胺酮为单次给药,氟西汀为连续21d给药。结果:产后3周,与空白对照组相比,孕前应激诱导的产后抑郁组模型母鼠在糖水偏好测试中的蔗糖消耗百分比显著降低(P<0.001),在强迫游泳测试和悬尾测试中绝望状态不动时间显著增加(P<0.01,P<0.01),在陌生环境摄食实验中摄食潜伏时间明显增加而单位时间摄食量显著减少(P<0.01,P<0.01)。单次给予YJ和氯胺酮24h之后,模型组母鼠的糖水消耗百分比显著增加(P<0.01),强迫游泳和悬尾测试的绝望不动时间明显降低(P<0.01,P<0.01),陌生环境摄食测试中潜伏时间显著缩短且单位时间摄食量明显增加(P<0.01,P<0.01),而氟西汀组未显示相同作用。结论:氟西汀连续给药对于孕前应激诱导的PPD模型小鼠无明显治疗作用,提示该模型可能属于难治性抑郁模型;在此模型上,越鞠丸具有与西药氯胺酮一致的快速抗抑郁作用。Objective：To establish a postpartum depression animal model,assess the abnormal maternal behavior of depressive dams induced by pre-pregnancy stress;to observe the rapid antidepressant effects of the single Yueju（YJ,a traditional Chinese medicine）ethanol extract,acute ketamine（Ket）and chronic fluoxetine（Flx,served as control）.Methods：Forty female BALB/c mice were randomly assigned to two groups,the control group and the pre-pregnancy stressed model groupfor 3weeks＇ chronic restraint stress.After the last stressor,the model group was housed with a male.Then at the end of 3weeks postpartum,we tested the maternal depressive-like behavior,including sucrose preference test（SPT）,forced swimming test（FST）,tail suspension test（TST）,and novelty suppressed feeding test（NSF）.Both YJand Ket were single administered24 hours before behavior test.While Flx was administrated once daily intraperitoneally（i.p）for21days,starting on postpartum day 1.Results：After 3weeks postpartum,Mod showed depressive behaviors.Reduced preference in drinking sucrose solution were found in SPT（P〈0.01）.Immobility in FST and TST were significantly increased（P〈0.01,P〈0.01）.In NSF,Mod displayed a significantly increase in latency and reduction in food consumption,compared with controls（P〈0.01,P〈0.01）.Acute YJimproved performance in SPT（P〈0.01）,FST（P〈0.01）,and NSF（P〈0.01,P〈0.01）,which was similar to Ket.While chronic Flx treatment,which was a SSRIS,could not change depressive behaviors of PPD mice.Conclusion：Chronic Flx did not improve depressive performance on PPD model induced by pre-pregnancy stress,indicating it＇s a refractory-like depression model.Acute YJexerted fast antidepressant effect on the PPD model similar to Ket.

关 键 词：产后抑郁 孕前应激 越鞠丸 快速抗抑郁 氯胺酮 

分 类 号：R749.053[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]