抑郁大鼠海马组织的比较蛋白质组学研究  

Analysis of CUMS-induced differentially expressed proteins in rat hippocampus by ITRAQ

作　　者：曹莉莎[1] 张芳[1] 罗文[1] 陈静[1] 何淼泉[1] 王继生[1] 

机构地区：[1]绵阳市第三人民医院临床药学科,四川绵阳621000

出　　处：《中国药理学通报》2016年第5期697-702,共6页Chinese Pharmacological Bulletin

基　　金：四川省卫生厅科研课题(No 120320)

摘　　要：目的比较慢性不可预见性温和应激(chronic unpredictable mild stress,CUMS)大鼠与健康大鼠的海马组织,筛选差异表达蛋白质。以期从蛋白质组学角度阐明其发病机制,并为寻找生物标志物提供靶标。方法采用CUMS建立大鼠抑郁模型。运用8标ITRAQ技术,结合2D LC-MS/MS分析CUMS大鼠与健康大鼠的海马组织差异表达蛋白质。结果共鉴定出5 109个蛋白,差异蛋白33个,其中8个蛋白表达上调,25个蛋白表达下调。结论 ITRAQ结合LCMS/MS技术能快速有效地进行蛋白质组学研究,为我们研究抑郁症的发生、发展机制以及发现新的生物标志物提供了有力平台。Aims To compare hippocampus between CUMS rats and normal rats, to find differentially ex-pressed proteins and to explore the pathogenesis of de-pression in the protein levels and biological marker. Methods Chronic unpredicted mild stress was taken to establish rat depression model. The ITRAQ-labeled proteins and peptides were separated by the cation col-umn, and differentially expressed proteins were detec-ted and identified by 2D LC-MS/MS. The functions of proteins were analyzed by bioinformatics. Results To-tally 5 109 proteins were identified, 33 differentially expressed proteins were identified, the expressions of 8 proteins were increased and 25 proteins downregulated. Conclusion ITRAQ based sereening is effective in discovering the nosogenesis of depression and new bio-logical marker.

关 键 词：ITRAQ 蛋白质组学 抑郁 海马组织 质谱 CUMS 

分 类 号：R394[医药卫生—医学遗传学]