不同过敏体质母亲母乳中PUFAs与免疫因子的差异及相互性研究  

Relationship and differences of polyunsaturated fatty acids and cytokines in human breast milk between allergic and non-allergic mothers

作　　者：邓莎莎[1] 陈铮[1] 彭咏梅[1] 

机构地区：[1]复旦大学附属儿科医院儿童保健科,上海201102

出　　处：《中国儿童保健杂志》2016年第4期344-349,共6页Chinese Journal of Child Health Care

基　　金：国家自然科学基金(81273087)

摘　　要：目的探讨不同过敏体质母亲母乳中多不饱和脂肪酸(PUFAs)和免疫因子的改变,同时分析体内多不饱和脂肪酸组成对免疫因子的影响。方法随机选取符合标准的80例母亲进入课题,产后2周和4周收集母乳,问卷调查母亲过敏史。采用高效毛细气相色谱分析技术检测母乳中脂肪酸浓度,分别采用酶联免疫吸附法和BioPlex悬液芯片技术测定母乳中免疫因子sCD14(soluble CD14)、IgE和IL-1β(interleukin-1β)、Eotaxin浓度。结果过敏性皮炎母亲2周母乳ALA(α-linolenic acid,ALA)和n-6PUFAs代谢系数(n-6LCPUFAs/LA)低于非过敏母亲,2周和4周母乳中LA/ALA(linoleic acid,LA)均高于非过敏母亲(P<0.05)。过敏性鼻炎母亲2周母乳ALA、EPA(eicosapentaenoic acid,EPA)、DHA(docosahexaenoic acid,DHA)含量均低于非过敏母亲(P<0.05)。无论过敏母亲还是非过敏母亲,母乳中多种n-3PUFAs(ALA、EPA、C20:3n-3)与sCD14呈正相关,与IgE负相关(P<0.05);母乳中多种n-6PUFAs(C18:3n-6,C20:3n-6,C22:2n-6)与sCD14呈负相关,与Eotaxin和IL-1β正相关(P<0.05)。结论不同过敏体质母亲与非过敏母亲母乳多不饱和脂肪酸组成存在差异;体内多不饱和脂肪酸组成对免疫因子的分泌具有重要影响。Objective To investigate the differences of human breast milk polyunsaturated fatty acids（PUFAs）and cytokines between mothers with and without allergic diseases,and to analyse the relationship between human breast milk PUFAs and immune factors. Methods A total of 80 breastfeeding mothers were recruited in this study.Breast milk samples at 2week and 4week postpartum were collected.Maternal allergic history were investigated by questionnaire.Fatty acid concentrations in breast milk were analyzed by gas chromatography.soluble CD14（sCD14）and IgE were analyzed by ELISA,while interleukin-1β（IL-1β）and Eotaxin were analyzed by BioPlex suspension array technique. Results Theα-linolenic acid（ALA）and n-6PUFAs metabolic index（n-6LCPUFA/LA）in 2weeks＇ breast milk of mothers with allergic dermatitis were lower than breast milk of mothers without allergy,but breast milk＇s LA/ALA（linoleic acid,LA）both in 2weeks and 4weeks were higher than breast milk of mothers without allergy（P〈0.05）.ALA,eicosapentaenoic acid（EPA）,docosahexaenoic acid（DHA）in 2week＇s breast milk of mothers with rhinitis were lower than breast milk of mothers without allergy（P〈0.05）.Regardless of allergic and non-allergic mother,many different kinds of n-3PUFAs（ALA,EPA,C20：3n-3）were positively related to sCD14 but negatively related to the IgE（P〈0.05）.Many different kinds of n-6PUFAs（C18：3n-6,C20：3n-6,C22：2n-6）were negatively correlated sCD14 but positively related to Eotaxin and IL-1β（P〈0.05）. Conclusions There are some differences in breast milk polyunsaturated fatty acids between allergic and non-allergic mothers.The concentrations of body polyunsaturated fatty acids have an important influence on the secretion of immune factors.

关 键 词：母乳 多不饱和脂肪酸 免疫因子 过敏性疾病 

分 类 号：R179[医药卫生—妇幼卫生保健]