生产虾青素的雨生红球藻藻株及其培养基筛选  

Selection of Haematococcus pluvialis for astaxanthin production and its culture medium optimization

作　　者：吴晓娟 唐小平 苏艳秋 罗国强 

机构地区：[1]通威股份有限公司,四川成都610041

出　　处：《中国饲料》2016年第8期29-32,共4页China Feed

基　　金：通威股份科研项目专项资金(TW2013D005;TW2015N010)

摘　　要：为筛选获得适合大规模生产虾青素的藻株及适宜其生长和虾青素积累的培养基，研究比较了3个不同品系的雨生红球藻H2、H3、H4在4种不同培养基BBM、BBM＋1g／LNaAC、SM、BGl1中的生长和虾青素积累情况。结果表明：SM有利于雨生红球藻的生长和生物量的积累：而在相同培养基中，生长速度为H3〉H2〉H4。在所有试验组中，藻株H3在培养基SM中营养生长和生物量积累最快，在培养基BBM＋1g／LNaAC中虾青素含量（2．17％）和虾青素产量（30．1mg／L）最高，分别为其他试验组的1．16～4．72倍（P〈0．05）和1．18～6．28倍（P〈0．01）。因此，藻株H3比较适宜作为大规模培养的藻株，其最适生长繁殖和虾青素积累的培养基分别为SM和BBM＋1g／LNaACn,In order to gain the suitable algae and culture medium for astaxanthin production,the influence of culture medium on the cell growth and astaxanthin accumulation of Haematococcus pluvialis were studied and compared.Three strains of Haematacoccus pluvialis(H2,H3 and H4) were cultured in four different media(BBM,BBM+1 g/L Na AC,SM and BG11).The results showed that the SM medium was benefit for the growth and biomass accumulation of Haematacoccus pluvialis.In the same medium,the growth rate was faster in H3 than H2 and H4.In all experimental groups,the strain H3 had the fastest growth rate and biomass accumulation rate in the medium SM,and had the highest astaxanthin content(2.17%)and astaxanthin yield(30.1 mg/L)in BBM+1 g/L Na AC medium,reaching 1.16 ~ 4.72 times(P < 0.05)and 1.18 ~6.28 times(P < 0.01)of those of other experimental groups,respectively.So,the strain H3 was fit for large scale cultivation,and its optimal medium for growth and astaxanthin accumulation were SM and BBM+1 g/L Na AC,respectively.

关 键 词：雨生红球藻 培养基 干重 虾青素 筛选 

分 类 号：S963[农业科学—水产养殖]