ERCC5基因miRNA结合部位多态性rs4150390与乳腺癌的易感性分析  

Analysis between Mi RNA Binding Site Polymorphism rs4150390 of ERCC5 and the Susceptibility of Breast Cancer

作　　者：郭欢[1] 郭红云[1] 李永辉[1] 王涛[1] 杨碎胜[1] 朱公建[1] 闵建平[1] 王海涛[1] 王兰[1] 张永东[1] 李海宁[1] 梁涛[1] 苏海翔[1] 

机构地区：[1]甘肃省医学科学研究院,甘肃省肿瘤医院,兰州730050

出　　处：《基因组学与应用生物学》2016年第3期479-483,共5页Genomics and Applied Biology

基　　金：甘肃省科技厅支撑项目(1011FKCA089);甘肃省卫生行业计划项目(GSWST2010-08)共同资助

摘　　要：本研究旨在探讨切除修复交叉互补基因5（complementation group 5,ERCC5）基因miRNAs结合位点的单核苷酸多态性（single nucleotide polymorphisms,SNPs）与甘肃地区女性乳腺癌易感性间的关系。以甘肃省散发乳腺癌病例101人和乳腺纤维腺瘤病例101人做研究对象;通过在线软件预测的方法,筛选出ERCC5基因rs4150390 SNP（G〉A）为研究位点,利用PCR-RFLP方法进行基因组DNA rs4150390位点SNP分型分析。研究结果显示rs4150390 A/G基因SNP与甘肃地区女性乳腺癌易感性无相关性,并且和肿瘤组织ER、PR、Her-2、P53的表达也无相关性。但当乳腺癌患者携带rs4150390突变型基因A/A时与肿瘤组织Ki67阴性表达相关（odds ratio,4.947;95%confidence interval,3.311~7.393;p=0.046）。综上所述rs4150390 A/G基因多态性与甘肃地区女性乳腺癌易感性无相关性。This study aims to evaluate the association between Single Nucleotide Polymorphisms（SNPs） in mi RNA binding site of excision repair cross complementing 5（ERCC5） gene and breast cancer susceptibility in women in Gansu province. 101 sporadic breast cancer cases and 101 breast fibroadenoma controls from Gansu province were enrolled;the SNP site rs4150390（GA） of ERCC5 was selected by the online software;the genotyping was conducted by PCR-RFLP method. The results revealed that there was no significant association between the polymorphisms in rs4150390 A/G and breast cancer risk in Gansu women,and there was no interaction between the SNP genotype and quantities of ER,PR,Her-2 and P53 in breast cancer tissue. Only an association was observed between ERCC5 rs4150390 A/A mutation carriers and Ki67 negative breast cancer cases（odds ratio,4.947;95% confidence interval,3.311~7.393;p =0.046）. This study provides evidence that no association between SNP rs4150390 A/G SNPs and the risk of breast cancer in women in Gansu province.

关 键 词：乳腺癌 核苷酸切除修复 ERCC5 SNP rs4150390 Ki67MiRNA 

分 类 号：R737.9[医药卫生—肿瘤]