红桂木凝集素受体在胃癌组织中的表达及其与临床病理相关性分析  

Expression of Artocarpus lingnanensis Lectin Receptors in Gastric Cancer and Its Association with Clinicopathological Characteristics

作　　者：尹利君[1] 廖烈君 刘康海[2] 朱其芳[1] 黄浩轩[1] 曾麒燕[1,3] 

机构地区：[1]广西医科大学生物化学与分子生物学教研室,南宁530021 [2]广西玉林市第一人民医院,玉林537000 [3]广西高校生物分子医学研究重点实验室,南宁530021

出　　处：《基因组学与应用生物学》2016年第3期484-489,共6页Genomics and Applied Biology

基　　金：国家自然科学基金(No.81160366);广西科技计划项目(桂科攻10124001A-23)共同资助

摘　　要：为探索胃癌的发生发展机制以及红桂木凝集素(Artocarpus lingnanensis lectin,ALL)在肿瘤诊断中的应用价值,本试验将红桂木凝集素进行辣根过氧化物酶或异硫氰酸荧光素标记,通过凝集素亲和组化法,检测胃癌组织和胃正常组织中红桂木凝集素受体表达的差异,结果发现胃癌组织经辣根过氧化物酶或异硫氰酸荧光素标记的红桂木凝集素(ALL-HRP或ALL-FITC)染色后,红桂木凝集素受体表达阳性率分别为75%(24/32)和68.8%(22/32),而正常胃组织的红桂木受体表达阳性率分别只有16%(4/25)和24%(6/25),胃癌和胃正常组之间差异有统计学意义(p<0.05),胃癌组织受体表达明显高于胃正常组织。红桂木凝集素受体表达与患者性别、年龄、分化程度、肿块大小、淋巴结转移、远处转移无相关性(p>0.05)。To investiga te the development mechanism of gastric cancer and explore the value of Artocarpus lingnanensis lectin（ALL） in tumor diagnosis,ALL was labeled with horseradish peroxidase or fluorescein isothiocyanate,and the expression of ALL receptors was observed in gastric cancer tissue and normal gastric tissue respe-ctively by lectin histochemistry. For ALL-HRP staining,ALL receptors were expressed in 24（75%） gastric cancer specimens,and 4（16%） cases in nomal gastric specimens. For ALL-FITC staining,ALL receptors were expressed in 22（68.8%） gastric cancer specimens,and 6（24%） cases in nomal gastric specimens. There was significantly different between gastric cancer tissue and nomal gastric tissue（p〈0.05）. The receptors expression of gastric cancer tissues was significantly higher than that of normal gastric tissues. However,there was no correlation with gender,ages,defferentiation degree,tumor size,lymph node metastasis,and distant metastasis（p〉0.05）.

关 键 词：红桂木凝集素 胃癌 受体 FITC HRP 

分 类 号：R735.2[医药卫生—肿瘤]