利用胞浆内单精子注射技术研究水牛与猪的种间受精:阶段性报告  

Study of Buffalo-Pig Inter-Species Fertilization by Intracytoplasmic Sperm Injection Technique: A Primary Report

作　　者：全守能 朱向星[1,2] 聂骏宇 肖鹏[1,2] 王雪芳[1,2] 许惠艳[1,2] 杨小淦[1,2] 陆阳清[1,2] 卢克焕[1,2] 卢晟盛[1,2] 

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室/广西高校动物繁殖与动物生物技术重点实验室,南宁530004 [2]广西大学动物科学技术学院,南宁530004 [3]贵港市人民医院生殖医学中心,贵港537100

出　　处：《基因组学与应用生物学》2016年第3期579-586,共8页Genomics and Applied Biology

基　　金：国家自然科学基金资助项目(31260553);广西硕士研究生创新课题(YCSZ2013003)共同资助

摘　　要：胞浆内单精子注射(intracytoplasmic sperm injection,ICSI)是一种研究种间受精机制的有效工具。本文旨在采用ICSI方法来构建水牛-猪种间显微授精卵,探讨了水牛-猪种间显微授精卵早期发育并检验了几种提高其发育潜力的方法。研究表明:(1)经ICSI方法制备的水牛-猪种间显微授精卵可以形成双原核,并能够发育到囊胚期;(2)PZM-3相对于SOF而言要更适于水牛-猪种间ICSI胚胎的培养;(3)用甲基化酶抑制剂5-azacytidine(5-Aza C)对水牛精子进行处理可以在一定程度上提高水牛-猪种间ICSI卵的原核形成率,但并不能提高其发育潜力。综上,本研究可为进一步探索水牛-猪种间受精机制及精卵互作奠定基础。Intracytoplasmic sperm injection（ICSI） is an effective tool for investigating the mechanism of interspecies fertilization. The present study was conducted to generate inter-species fertilized oocytes by using of buffalo sperms and porcine oocytes via ICSI. In this study,we studied the early development of buffalo-pig inter-species ICSI fertilized oocytes（termed as buffalo-pig ICSI oocytes）,and also examined some methods used for improving the development of such oocytes. We found that（1） the 2 pronucleus formation was indeed occured in these buffalo-pig ICSI oocytes generated via ICSI,and such oocytes could develop to the blastocyst stage;（2） the PZM-3 was examined be more suitable than SOF for culturing buffalo-pig ICSI oocytes;（3） we comfirmed that the DNA methyltransferases（DNMTS）,5-azacytidine（5-Aza C）,was able to improve,at a certain extent,the 2 pronucleus formation in buffalo-pig ICSI oocytes,whereas with no significant effect on the in vitro development.Summarily,this study lay the foundation for further investigating the fertilized mechanisms of buffalo-pig inter-species fertilization and sperm-oocyte interaction.

关 键 词：水牛 猪 卵母细胞 精子 种间受精 种间显微授精 胞质内单精子注射 

分 类 号：S828[农业科学—畜牧学] S823.83[农业科学—畜牧兽医]