响应面法优化灵芝蛋白质提取工艺  

Response Surface Optimization of Protein Extraction from Ganoderma Lucidum

作　　者：陈濠[1] 贾瑞博[1] 范锦琳 陈艺煊 王莹[1] 陈紫红[1] 赵立娜 刘斌[1,2] 

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]国家菌草工程技术研究中心,福建福州350002

出　　处：《农产品加工》2016年第3期33-36,39,共5页Farm Products Processing

基　　金：国家自然科学基金青年基金项目(31501432)

摘　　要：以灵芝蛋白质的提取率为指标,考察液料比(V/W)、时间、温度及p H值对提取率的影响,在单因素试验的基础上,采用响应面分析方法确定灵芝蛋白质的最优提取工艺。结果表明,灵芝蛋白质最佳提取工艺条件为液料比(V/W)120∶1,温度50℃,p H值10,时间60 min,沉淀p H值3,在此优化工艺下灵芝粗蛋白的实际提取率达39.7%。The protein extraction rate is taken as index to study the effect of ratio of material：liquid- material ratio（V/W）,extraction time, temperature and p H on extraction yield. On the basis of single factor experiments, the optimized protein extraction process is analyzed by response surface method. The results show that the optimal Ganoderma lucidum protein extraction conditions are as follows： liquid- material ratio（V/W） of 120 ∶1, extraction temperature of 50 ℃, p H of 10,extraction time of 60 min,and precipitation p H of 3. And the real extraction ratio obtained by these optimized conditions is 39.7%.

关 键 词：灵芝蛋白质 响应面分析法 提取工艺 

分 类 号：TQ464.7[化学工程—制药化工]