壮药了刁竹含量测定方法的建立  

DETERMINATION OF THE CONTENT OF CYNANCHUM PANICULATUM(BGE.)KITAG.OF ZHUANG MEDICINE

作　　者：黄琴[1] 蒋伟哲[2] 黄瑞松 陆峥琳 叶积秀 

机构地区：[1]中国人民解放军第303医院 [2]广西医科大学药学院,南宁530021 [3]广西民族医药研究院,南宁530021 [4]广西中医药大学药学院,南宁530021

出　　处：《广西医科大学学报》2016年第1期153-156,共4页Journal of Guangxi Medical University

基　　金：广西科学研究与技术开发计划项目重大专项计划资助项目(No.桂科重1355001-4);广西医疗卫生重点科研课题资助项目(No.重2012015)

摘　　要：目的:建立了刁竹丹皮酚含量测定方法,为控制该药材质量提供技术支撑。方法:对HPLC的色谱柱、流动相、检测波长等色谱条件进行摸索,采用正交设计的方法对供试品溶液的制备条件进行筛选。结果:建立的含量测定方法色谱条件:色谱柱为ZORBAX SB-C18柱(4.6 mm×150 mm,5μm,柱温25℃),流动相为甲醇∶水(55∶45),流速1.0 mL/min,检测波长274nm;供试品溶液制备条件:药材破碎度为过三号筛的粉末,提取溶剂为甲醇,溶剂加入量25mL,回流时间30min。结论:该测定方法简便、准确、重现性好,适用于了刁竹药材的质量控制。Objective： To investigate method for content determination of paeonol of Cynanchum paniculatum （Bge.） Kitag. , and to provide technical support for quality control of the medicinal material. Methods：Comparing different chromatographic column, flow velocity, detection wavelength to confirm the chromatographic conditions. Orthogonal design was applied to study the samples＇ preparation conditions. Results：The chromategraphic column was Agilent ZORBAX SB-C18 （4.6 mm× 150 mm, 5 μm） ; themobile phase was methanol ： water （55 ： 45） with the flow of 1.0 mL/min, and the detection wavelength was 274 nm. The preparation condition of sample solution was as follow：the pulverization degree of medicinal materials was over No. 3 sieve powder, methanol amount was of 25 mL, and the reflux time was 30 rains. Conclusion： The established method is accurate, reliable and reproducible, which might be suitable for quality control of the medicinal materials.

关 键 词：了刁竹 丹皮酚 含量测定方法 

分 类 号：R-33[医药卫生]