冰冻红细胞解冻洗涤液的改良性研究  

Exploration on the Improvement of thawed Washing Solution of Frozen Red Blood Cells

作　　者：于来水[1] 康炜[1] 宫本兰[1] 叶萍[1] 张薇[1] 张磊[1] 梁晓华[1] 

机构地区：[1]辽宁省大连市血液中心,116001

出　　处：《临床输血与检验》2016年第2期149-152,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9%Na Cl)和第2次洗涤液(200 ml 0.9%Na Cl)中分别加入终浓度为1.2%、0.2%的木糖醇,比较两组的RBC回收率、红细胞渗透脆性、甘油残留量,同时测定冰冻红细胞解冻洗涤过程中各阶段的游离血红蛋白含量,明确提高解冻红细胞回收率的关键步骤。结果实验组RBC回收率显著高于对照组(t=-10.222,P<0.05),而红细胞渗透脆性、甘油残留量与对照组相比差异无统计学意义(P>0.05)。解冻第1次洗涤后血浆游离血红蛋白含量以实验组显著低于对照组(t=24.496,P<0.05),其余洗涤步骤的差异则无统计学意义(P>0.05)。结论冰冻红细胞经第1次洗涤液(80 ml 9%Na Cl)的解冻洗涤过程为RBC回收率提高的关键步骤。Objective To explore the feasibility of improving the recovery ratio of red blood cells in frozen- thawed red blood ceils by adding an appropriate amount ofxylitol during the washing process. Methods 20 packs of 400mL red blood cells which were leukocyte-reduced and prepared within 6 days were selected. The plasma was removed after centrifuge and each pack was divided into 2 bags （100 ml each） as experimental group and control group. In the experimental group, xylitol with final concentration of 1.2%,0.2% was added into first washing solution （80 ml 9% NaCl） and second washing solution （200 ml 0.9% NaCl）.The recovery ratio of red blood cells, residue level of glycerin and osmotic fragility in two groups were statisticaly analyzed. The key step of improving recovery ratio of red blood cells was sought by detecting the concentrate of free hemoglobin in washing process. Results The recovery ratio of red blood cells was significantly higher in experimental group than that in control group （t=-10.222,P〈0 .05） while the concentration of free hemoglobin after first washing was significantly lowered （t=24.496,P〈0 .05） . No significant difference was seen in the residue of glycerin,osmotic fragility and free hemoglobin after additional washing （P〉 0.05） . Conclusion The key step of improving recovery ratio of frozen-thawed red blood cells was the first washing process.

关 键 词：冰冻红细胞 木糖醇 洗涤液 

分 类 号：R331.141[医药卫生—人体生理学]