携带诺帝的聚乙烯醇颗粒治疗兔VX-2肝移植肿瘤的疗效研究  

Intra-tumoral injection with polyvinyl alcohol particles containing nordy for treatment of transplanted vx-2 tumor in rabbits

作　　者：何学红[1] 游箭[1] 蒲洪波[1] 陈竹[1] 李蓉芬[1] 

机构地区：[1]川北医学院第二临床医学院.南充市中心医院影像科/介入放射科,四川南充637000

出　　处：《西部医学》2016年第4期461-464,共4页Medical Journal of West China

基　　金：四川省教育厅重点项目(11ZA188);南充市科技支撑项目(11A0118)

摘　　要：目的探讨经导管肝动脉注射携带诺帝的PVA颗粒治疗兔VX2肝移植肿瘤的短期疗效。方法采用开腹直接种植法制备兔VX2肝移植肿瘤模型;随机选出30只VX2瘤兔进行介入治疗,分为对照组(注射生理盐水5ml)、TACE组(注射碘化油乳剂0.2ml/kg+顺铂1mg/kg)、实验组(注射携带诺帝的PVA颗粒1mg/kg)三组,每组10只;术前1d及术后1、3、7d观察血清谷丙转氨酶(ALT)、谷草转氨酶(AST)及肌酐(Cr)水平的变化,术前及术后7、14d分别行CT扫描,测量肿瘤大小计算肿瘤体积增长率、统计转移部位;术后7d采用ELISA方法检测血清VEGF蛋白含量。结果TACE组、实验组术后1、3d血清ALT、AST显著升高,两组无明显差异,但与对照组相比有显著性差异(P<0.01),两组术后1、3d血清Cr没有显著性差异;ALT、AST、Cr术后7d基本降至术前水平;TACE组、实验组术后14d肿瘤体积增长率均小于对照组,差异显著(P=0.000),同时实验组肿瘤体积增长率低于TACE组,也有显著性差异,转移部位少于TACE组;术后7d血清VEGF蛋白含量以TACE组最高,与对照组、实验组相比,具有显著性差异;实验组高于对照组,也有显著性差异。结论携带诺帝的PVA颗粒短期内具有一定的抑制肿瘤生长效果,可为临床治疗肿瘤提供一种新的思路。Objective To explore the short-term effect of PVA particles loding nordy perfusion via hepatic artery for rabbit VX2 liver tumor.Methods The methods of cutting the belly open was used to make the transplanted model of VX2 carcinoma in rabbit.30 rabbits VX2 with liver tumor model were divided into control group（injected with 5 ml NS）,TACE group（injected with 0.2ml/kg lipiodol and 1mg/kg cisplatin）,experimental group（injected with 1mg/kg PVA particles which combined with nordy）.The changes of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）and creatinine（Cr）at 1day before and 1,3,7day after treatment,the blood supply,necrosis and metastasis of tumor tissue were observed by CT before and 7,14 day after treatment,the max and minimum diameter of tumor and the tumor volume growth rate,tumor metastasis and the VEGF at 7day after treatment were observed.Results The AST and ALT at 1day and 3day after treatment in the TACE group and the experimental group were significant higher than that of the control group（P〈0.01）.But Cr was no significant change at 1th day and 3th day after treatment.The three indexes decreased to the level pre-treatment at 7day after treatment.The tumor volume growth rate in the two groups were less than that of control group at 14 day after treatment（P=0.000）.The tumor volume growth rate and tumor metastasis of experimental group were less than that of TACE group,and the difference was significant.The VEGF was the highest in TACE group,and there existed significant difference compared with the other two groups.This index in experimental group was higher than control group.Conclusion The treatment for rabbit VX-2liver tumor with PVA microspheres loding nordy has short-term effect of inhibitory action on tumor growth and provides a new method for the treatment of tumor.

关 键 词：诺帝 VX2肿瘤 介入治疗 血管内皮生长因子 肿瘤体积增长率 

分 类 号：R735.7[医药卫生—肿瘤]