医用臭氧对肝癌细胞HepG2生物学行为的实验研究  

Research for the treatment of medical ozone on liver cancer cell line HepG2

作　　者：周玉川[1] 刘康[1] 钟立明[2] 杨巧丽[2] 游箭[2] 魏欣[2] 邓川[2] 

机构地区：[1]川北医学院第二临床医学院.南充市中心医院组织工程与干细胞研究所,四川南充637000 [2]川北医学院第二临床医学院.南充市中心医院介入放射科,四川南充637000

出　　处：《西部医学》2016年第4期465-468,共4页Medical Journal of West China

基　　金：南充市应用技术研究与开发项目(13A0047)

摘　　要：目的探讨使用医用臭氧(O3)在体外对肝癌HepG2细胞的作用。方法获取人肝癌HepG2细胞在体外增殖、传代、对数生长期的细胞,将细胞分为对照组和实验组,实验组采用含浓度为0.5μg/ml的医用臭氧水的培养液处理细胞,对比两组试验的细胞形态学变化,细胞生长曲线、细胞凋亡情况、细胞迁移能力变化。结果体外实验结果显示,含臭氧的培养基中细胞数量明显减少,肿瘤细胞膜破裂,核固缩甚至裂解,臭氧对细胞的生长有抑制作用,同时CCK-8的检测结果显示O3能抑制肝癌细胞的增殖(P<0.01),Transwell迁移实验显示O3能抑制肿瘤细胞的迁移(P<0.01)。结论臭氧能直接杀死肝癌HepG2细胞,能在体外抑制肝癌HepG2细胞的增殖和迁移。Objective To explore the inhibitory effect of medical ozone on liver cancer cell line HepG2 in vitro.Methods The liver cancer cell line HepG2 were divided into experiment group and control group.The experiment group was treated with 0.5μg/ml medical ozone.The morphology,growth curve,apoptosis and migration ability were observed.Results The cells in experiment group were decreased compared with that of control group.There were cell membrane breakage,karyopycnosis and even nuclear cracked in experiment group.Transwell model Assay showed tumor cells migration was inhibited by ozone solution.Conclusion Ozone can restrain proliferation and migration of liver cancer cell line HepG2,and can directly kill HepG2 liver cancer cells in vitro.

关 键 词：臭氧 肝癌HEPG2细胞 抑制作用 杀伤 

分 类 号：R735.7[医药卫生—肿瘤]