肝癌患者血清中共刺激分子sB7-H3和炎症因子IL-17、IL-8相关性及临床诊断研究  

Correlations between the soluble form of B7-H3 and the cytokines of IL-17 and IL-8 in patients with primary hepatocellular carcinoma and their clinical values for early diagnosis of hepatocellular carci-noma

作　　者：李广云[1] 陈政华[2] 徐逢皇 王雪丹[1] 王斐斐[2] 易君祝 栾希英[3] 

机构地区：[1]山东省千佛山医院,济南250014 [2]滨州医学院烟台附属医院,烟台264100 [3]滨州医学院免疫学教研室,烟台264003

出　　处：《中华微生物学和免疫学杂志》2016年第3期213-218,共6页Chinese Journal of Microbiology and Immunology

基　　金：山东省医药卫生科技发展计划项目（2013WS0132）;滨州医学院科技发展计划（BY2013KJ48）

摘　　要：目的 研究肝癌患者血清中共刺激分子sB7-H3与炎症因子IL-17、IL-8的相关性及其在肝癌临床诊断中的应用价值.方法 收集原发性肝细胞癌患者的血清标本,以同期健康体检人员外周血标本作为正常对照.采用ELISA法检测肝癌组和正常对照组血清中sB7-H3、IL-8、IL-17的水平;运用受试者工作特征曲线（receiver operating characteristic curve,ROC）统计学分析比较三者对肝癌的诊断效能,logistic回归获得sB7-H3、IL-8、IL-17三者联合诊断的模型预测肝癌.结果 原发性肝癌患者血清中sB7-H3、IL-17和IL-8的含量均明显高于正常对照组.sB7-H3与IL-17呈正相关,但与IL-8不相关.IL-8与IL-17之间呈负相关.ROC分析sB7-H3、IL-17和IL-8预测肝癌的AUC值分别为0.832、0.657和0.953,对诊断肝癌三者差异均有统计学意义.通过Logistic逐步回归得出的回归模型预测肝癌的曲线下面积（area under the curve,AUC）值、敏感度、特异度分别为0.960、91.30%和94.29%均较单独诊断时高.结论 原发性肝细胞癌患者外周血中sB7-H3与IL-17呈正相关,sB7-H3、IL-17和IL-8联合回归模型在肝癌的早期诊断中具有潜在的应用价值.Objective To investigate the correlations between the soluble form of B7-H3 （ sB7-H3） and the cytokines of IL-17 and IL-8 in serum samples from patients with primary hepatocellular carcino-ma （ HCC） and to evaluate their clinical values for early diagnosis of HCC.Methods Serum samples were collected from 63 patients with HCC and 50 healthy subjects.The expression of sB7-H3, IL-17 and IL-8 in serum samples were detected by ELISA.Receiver operating characteristic （ ROC） curve was generated to an-alyze the diagnostic values of sB7-H3, IL-17 and IL-8 for hepatoma.The logistic regression model was used to predict the probability of hepatoma by using sB7-H3, IL-17 and IL-8 in combination.Results The levels of sB7-H3, IL-17 and IL-8 in serum samples collected from the patients with HCC were significantly higher than those from healthy subjects.A positive correlation was found between the levels of sB7-H3 and IL-17 in serum samples from patients with HCC.No correlation was found between sB7-H3 and IL-8.A negative cor-relation was found between the levels of IL-17 and IL-8 in serum samples from patients with HCC.ROC curve analysis showed that the area under the curve （AUC） of sB7-H3, IL-17 and IL-8 were 0.832, 0.657 and 0.953, respectively, indicating the statistical significance of them for the diagnosis of HCC.The logistic regression showed that the AUC, diagnostic sensitivity and specificity of the regression model PRE in the pre-diction of HCC were 0.960, 91.30% and 94.29%, respectively, which was much better than using the three indicators alone.Conclusion The levels of sB7-H3 were positively correlated to the levels of IL-17 in serum samples from patient with HCC.The logistic regression model of combination of sB7-H3, IL-17 and IL-8 obtained in this study could be used for early clinical diagnosis of HCC in the future.

关 键 词：B7-H3 IL-17 IL-8 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]