β-catenin介导蛋白激酶D2对脑胶质瘤细胞增殖的影响  

Involvement of β-catenin in effect of protein kinase D2 on glioma cell proliferation

作　　者：宋旭[1] 王猛[2] 华磊[1] 陆冬[1] 石琼[3] 周秀萍[3] 于如同[1,3] 

机构地区：[1]徐州医学院附属医院脑科医院,徐州221002 [2]徐州医学院研究生学院 [3]徐州医学院神经系统疾病研究所

出　　处：《临床神经外科杂志》2016年第1期40-44,共5页Journal of Clinical Neurosurgery

基　　金：国家自然科学基金(81272777;81372699;81472345)

摘　　要：目的研究蛋白激酶(PKD2)是否通过β-catenin来调控脑胶质瘤细胞的增殖。方法采用EDU掺入实验观察下调PKD2或β-catenin后U251细胞增殖能力的变化;免疫印迹实验检测下调PKD2后β-catenin总蛋白水平及其在细胞膜、细胞浆及细胞核中的变化。结果与对照组相比,下调PKD2后U251细胞增殖能力降低;β-catenin总蛋白水平无明显变化,但细胞浆β-catenin水平明显增多,细胞核中明显减少,差异有统计学意义(均P<0.01)。与对照组相比,下调β-catenin后,U251细胞增殖能力降低,差异有统计学意义(P<0.01)。结论下调PKD2可能通过抑制β-catenin向细胞核转位而抑制脑胶质瘤细胞的增殖。Objective To study whether protein kinase（PKD2） affect glioma proliferation by regulating β-catenin.Methods The effect of PKD2 or β-catenin down-regulation on the proliferation of glioma cells was detected by EDU incorporation assay.Western blotting was applied to detect total β-catenin expression level and β-catenin level in each subcellular fraction,such as the membrane,nuclear and cytosolic fractions of the cells after down-regulation of PKD2.Results Compared with the control group,the proliferation of glioma cells decreased in PKD2down-regulation group（P 〈0.01）.The total β-catenin protein level did not change,but the cytosolic fractions increased,while those in the nuclear fraction decreased after PKD2 down-regulation（P〈 0.01）.Conclusion Down-regulation of PKD2 may inhibit glioma cell proliferation by decreasing β-catenin level in the nuclear fraction.

关 键 词：胶质瘤 蛋白激酶D2 细胞增殖 Β-连环蛋白 

分 类 号：R739.4[医药卫生—肿瘤]