miR-491通过下调Xklp2靶蛋白(TPX2)表达抑制肝癌细胞增殖、侵袭及迁移  

miR-491 inhibits the proliferation, invasion and migration of hepatocellular carcinoma cell via down-regulating TPX2 expression

作　　者：王军[1] 刘志奎[2] 窦常伟[2] 韩少山[2] 李超[2] 涂康生[2] 杨威[2] 

机构地区：[1]西安交通大学第一附属医院急诊科,陕西西安710061 [2]西安交通大学第一附属医院肝胆外科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2016年第4期512-517,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81402039);陕西省自然科学基础研究计划(2015JM8409)

摘　　要：目的探讨miR-491在肝癌组织中的表达、临床意义及可能的分子机制。方法实时定量PCR检测miR-491在肝癌及癌旁组织中的表达,MTT法检测MHCC-97H细胞的增殖,TranswellTM实验检测MHCC-97H细胞的侵袭及迁移,Western blot法及免疫组织化学染色检测miR-491下游潜在靶点Xklp2靶蛋白(TPX2)的表达。结果 miR-491在肝癌组织中表达水平显著低于相应癌旁组织;肝癌组织中miR-491低表达与肿瘤大小、TNM分期、门静脉侵犯及Edmondson分级显著相关;低表达miR-491患者3年生存率明显低于高表达组;miR-491可显著抑制MHCC-97H细胞的增殖、侵袭及迁移,并下调TPX2的蛋白水平;miR-491低表达组TPX2蛋白水平明显高于miR-491高表达组,相关性分析显示肝癌组织中miR-491与TPX2蛋白表达呈显著负相关。结论 miR-491在肝癌组织中表达下调并与肝癌恶性临床病理特征有关,miR-491抑制肝癌细胞增殖、侵袭及迁移的作用可能与下调TPX2表达有关。Objective To investigate the level and clinical significance of miR-491 expression in human hepatocellular carcinoma（ HCC） and the underlying mechanisms. Methods Real-time quantitative PCR was applied to detect the expression of miR-491 in HCC and the corresponding tumor-adjacent tissues. The proliferation of MHCC-97 H cells was tested by MTT assay. TranswellTMassay was performed to measure the invasion and migration of MHCC-97 H cells. The expression of targeting protein for Xklp2（ TPX2） was determined by Western blotting and immunohistochemistry. Results The expression level of miR-491 in HCC tissues was significantly lower than that in the adjacent normal tissues,and it was obviously associated with tumor size,TNM stage,portal vein infiltration and Edmondson degree. Patients in the lower miR-491 group had a worse 3-year survival than those in higher miR-491 group. Overexpression of miR-491 in MHCC-97 H cells markedly suppressed cell proliferation,invasion and migration,and downregulated the protein expression of TPX2. Correlation analysis showed that miR-491 level was negatively correlated with TPX2 expression level in HCC tissues. Conclusion The expression of miR-491 in HCC tissue is significantly lower than that in tumor-adjacent tissues and correlated with the malignant clinical pathological features. miR-491 inhibits HCC cell proliferation,invasion and migration by downregulating the expression of TPX2.

关 键 词：miR-491 肝细胞癌 增殖 侵袭 迁移 Xklp2靶蛋白(TPX2) 

分 类 号：R735.7[医药卫生—肿瘤] R392.33[医药卫生—临床医学]