腹泻型肠易激综合征大鼠模型的评价研究  

Evaluate a rat model of diarrhea-predominant pattern irritable bowel syndrome

作　　者：李培彩[1,2] 卢小芳[2] 张声生[2] 吴震宇[1,2] 王佳佳[2] 汪正芳[2] 杨成[2] 张恒钰[1,2] 

机构地区：[1]北京中医药大学,北京100029 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《中国中西医结合消化杂志》2016年第3期174-177,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金(No:81473644);北京市医院管理局临床医学发展专项经费资助(No:ZYLX201411);北京市卫生系统高层次卫生技术人才培养计划(No:2011-2-13)

摘　　要：[目的]对母婴分离联合束缚应激法建立的腹泻型肠易激综合征（Diarrhea-predominant pattern irritable bowel syndrome,D-IBS）大鼠模型进行评价。[方法]16只2日龄SD大鼠随机分为正常组（8只）和模型组（8只）。模型组大鼠第2-21日龄,每天将新生期大鼠与哺乳期母鼠分离3h;第50-59日龄每天予束缚应激刺激。正常组不予任何干预,直至大鼠60日龄。造模结束后,检测各组大鼠体重变化、粪便含水量、结肠球囊扩张刺激时大鼠腹壁撤退反射（Abdominal withdrawal reflex,AWR）评分及腹外斜肌肌电（Electromyography,EMG）积分变化率。[结果]模型组大鼠体重低于正常组（P〈0.05）,粪便含水量明显高于正常组（P〈0.05）;与正常组比较,在结肠气囊压力为40mmHg、60mmHg、80mmHg时,模型组大鼠AWR评分及EMG积分变化率均显著升高（P〈0.05）;2组大鼠结肠黏膜均无明显病理改变。[结论]采用母婴分离联合束缚应激的方法建立D-IBS大鼠模型可在一定程度上模拟D-IBS的腹泻症状及内脏高敏感的状态。[Objective]To evaluate a rat model of diarrhea-predominant pattern irritable bowel syndrome（D-IBS）resulting from the method of neonatal maternal separation and restraint stress.[Methods]Sixteen2-day-old Sprague-Dawley rats were randomly divided into two groups,control group（n=8）and model group（n=8）.Rats in the model group received maternal separation for 3hours in postnatal days 2-21.Rats in both groups were then followed to 50 days,at which point rats in model group received restraint stress for 10 days.Then the weight change,defecation,behavioral Abdominal Withdrawal Reflex（AWR）scores and electromyographic（EMG）recordings of the obliquus externus abdominis responses to graded balloon distention（20-80mmHg）were tested.[Results]The weight of model rats was significantly lower（P〈0.05）and waste water content（%）（P〈0.05）was significantly higher than the controls.Compared with the normal control group,Significant increases（P〈0.05）in the AWR scores and EMG activity of model rats were observed at balloon distention pressures of 40-80 mmHg.However,no significant colon pathology was found in both groups.[Conclusion]The method of neonatal maternal separation and restraint stress can establish a potential model which simulates clinical symptoms and visceral hypersensitivity of D-IBS.

关 键 词：肠易激综合征 母婴分离 束缚应激 内脏高敏感 

分 类 号：R574[医药卫生—消化系统]