野葛块根异黄酮的提取及抗氧化研究  

Extraction and antioxidant effects of isoflavone in Pueraria lobata( Willd. ) Ohwi root tuber

作　　者：李玲[1,2] 谭力[1] 全沁果 全浩[1] 何福林[1] 黄光文[1] 邵金华[1] 闫旭宇[1] 

机构地区：[1]湖南科技学院化学与生物工程学院,湘南优势植物资源综合利用湖南省重点实验室,湖南永州425199 [2]浙江大学农业与生物技术学院,浙江杭州310058 [3]中国农业科学院农产品加工研究所,农业部农产品加工质量安全风险评估实验室,北京100193

出　　处：《浙江农业学报》2016年第3期496-501,共6页Acta Agriculturae Zhejiangensis

基　　金：中国博士后科学基金面上项目(2014M561768);湖南科技学院湘南优势植物资源综合利用湖南省重点实验室开放基金(XNZW14C12);湖南省永州市2015年度指导性科技计划项目(2015-10);永州市科技创新项目(永财企指2015[25]号-9)

摘　　要：以土家山区野葛块根为试验材料,采用超声波辅助乙醇提取野葛块根异黄酮。在单因素试验的基础上,以提取率为响应值,运用4因素4水平响应面分析法研究提取过程中工艺参数对提取率的影响,并对异黄酮提取物的抗氧化性能进行了研究。结果表明:最适提取工艺条件为乙醇浓度81.0%,超声时间41.2min,提取温度61.2℃,料液比(m/V)1∶9.5,原料粒径40目。在此工艺条件下,野葛块根异黄酮的平均提取率为3.43%。所提取的野葛块根异黄酮的抗氧化效果低于Vc,但能与其产生一定的协同作用。In the present study,isoflavone was extracted from Pueraria lobata（ Willd.） Ohwi root tuber collected in Tujia Mountain by ultrasound-assisted ethanol extraction. Based on single factor test analysis,main technology parameters of the extraction process were optimized by response surface design of 4 factors and 4 levels,and antioxidant effects of the extracted isoflavone were also studied. The results showed that the optimum extraction technology was as follows： the ethanol concentration was 81. 0%,ultrasound-assisted extraction time was 41. 2 min,extraction temperature was 61. 2 ℃,solid to liquid ratio（ m / V） was 1∶ 9. 5,and particle size of raw material was 40 mesh. Under this condition,the extraction rate of isoflavone reached 3. 43%. The antioxidant effects of the extracted isoflavone were weaker than that of vitamin C,but it could yield synergetic effect with vitamin C.

关 键 词：野葛 异黄酮 抗氧化 

分 类 号：S58[农业科学—作物学] TQ464[化学工程—制药化工]