FBXW7过表达可抑制乳腺癌MCF-7细胞增殖和迁移并促进细胞凋亡  

Over-expression of FBXW7 inhibits proliferation and metastasis and promotes apoptosis of breast cancer MCF-7 cells

作　　者：滕牧洲 陈利娜[1] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515

出　　处：《肿瘤》2016年第4期388-395,共8页Tumor

摘　　要：目的:探讨F框/WD-40域蛋白7(F-box and WD-40 domain protein 7,FBXW 7)基因过表达对乳腺癌MCF-7细胞增殖、迁移、周期和凋亡的影响。方法:构建FBXW 7过表达的重组载体pEZ-M02-FBXW7质粒,并将其转染乳腺癌MCF-7细胞,应用实时荧光定量PCR和蛋白质印迹法检测pEZ-M02-FBXW7转染后MCF-7细胞中FBXW7 mRNA和蛋白的表达,CCK-8和Transwell法检测细胞增殖、迁移和侵袭情况,FCM法检测细胞周期和细胞凋亡情况。结果:pEZ-M02-FBXW7转染后,乳腺癌MCF-7细胞中FBXW7 mRNA和蛋白的表达水平高于阴性对照组(将空载体pEZ-M02质粒转染至MCF-7细胞)和空白对照组(未进行转染的MCF-7细胞)(P值均<0.01),MCF-7细胞的增殖、迁移和侵袭能力均明显下降(P值均<0.05),MCF-7细胞被阻滞于G0/G1期(P<0.05),且细胞凋亡率明显升高(P<0.05)。结论:FBXW7高表达可抑制乳腺癌MCF-7细胞的增殖、侵袭和迁移,并可促进细胞凋亡。Objective：To investigate the effects of over-expression of F-box and WD-40 domain protein 7（FBXW7） gene on the proliferation,metastasis,cell cycle and apoptosis of breast cancer MCF-7 cells.Methods：The recombination vector pEZ-M02-FBXW7 plasmid was established and transfected into breast cancer MCF-7 cells.The expressions of FBXW7 mRNA and protein in MCF-7 cells after transfection with pEZ-M02-FBXW7 were detected by real-time fluorescent quantitative PCR and Western blotting,respectively.The abilities of proliferation,migration and invasion of MCF-7 cells aftertransfection with pEZ-M02-FBXW7 were detected by CCK-8 and Transwell assay,respectively,and the cell cycle and apoptosis were determined by FCM.Results：The expression levels of FBXW7 mRNA and protein in breast cancer MCF-7 cells after transfection with pEZ-M02-FBXW7 were higher than those in negative control cells（MCF-7cells transfected with empty vector pEZ-M02） and blank control cells（MCF-7 cells without any transfection）（all P 〈0.01）.The abilities of proliferation,migration and invasion of MCF-7 cells after transfection with pEZ-M02-FBXW7 were significantly reduced（all P 〈0.05）,and the cell cycle was arrested at G0/G1 phase（P 〈0.05）,whereas the apoptotic rate was increased（P 〈0.05）.Conclusion：Over-expression of FBXW7 can inhibit the proliferation,invasion and migration of breast cancer MCF-7 cells,and it can also promote apoptosis.

关 键 词：乳腺肿瘤 细胞增殖 细胞运动 细胞凋亡 FBXW7基因 

分 类 号：R737.9[医药卫生—肿瘤]