锰暴露诱导BV2小胶质细胞环氧合酶2表达变化  

Expressional change of cyclooxygenase 2 induced by manganese exposure in microglia BV2 cells

作　　者：王晓静[1] 曹子鹏[1] 赵芳[1] 王迪雅[1] 苏鹏[1] 康蓓佩 骆文静[1] 

机构地区：[1]第四军医大学军事预防医学系劳动与环境卫生学教研室,陕西西安710032

出　　处：《实用预防医学》2016年第5期535-539,共5页Practical Preventive Medicine

基　　金：国家自然科学基金重点项目(81230063)

摘　　要：目的观察锰暴露诱导BV2小胶质细胞活化过程中COX2分子表达的变化。方法实验中BV2细胞分3组,为对照组,50μM锰暴露组和100μM锰暴露组,分别处理12、24和48 h。免疫荧光化学染色方法观察BV2细胞锰暴露24h后的活化情况,荧光实时定量PCR(qRT-PCR)和Western Blot法分别检测COX2分子mRNA和蛋白表达水平的改变。结果免疫荧光化学染色结果显示,锰暴露24 h后BV2小胶质细胞形态发生改变,细胞胞体变大变圆,突起变短或消失,活化细胞占总细胞数的比例增加(P<0.05);实时定量PCR结果显示,锰暴露12 h和24 h后COX2 mRNA水平增加(P<0.05);Western Blot结果显示,锰暴露24 h和48 h后COX2蛋白表达水平增加(P<0.05)。结论锰暴露能够诱导BV2细胞COX2分子表达增加,COX2可能参与了锰诱导小胶质细胞活化的过程。Objective To investigate the expressional change of cyclooxygenase 2 （COX2） in the activation of microglia BV2 cells induced by manganese exposure. Methods The BV2 cells in the experiments were divided into three groups, the control group, 50μM manganese exposure group and 100μM manganese exposure group. The treatment time was 12h, 24h and 48h. Immunofluorescence assay was applied to observe the activation of BV2 cells exposed to manganese for 24h. Fluorescence real- time quantitative PCR （qRT- PCR） and Western Blot were respectively performed to detect the quantitative change of COX2 in mRNA level and protein level. Results The morphology of BV2 cells changed when treated by manganese for 24h. Cell bod- ies became larger and round while the processes became shorter or disappeared. The proportion of activated cells increased （P 〈 0.05）. qRT- PCR showed that the COX2 mRNA was upregulated after 12h and 24h manganese exposure （P〈0.05）. West- em Blot demonstrated that the COX2 protein was upregulated after 24h and 48h manganese treatment （P 〈 0.05）. Conclu- sions Manganese exposure can increase the expression of COX2 in BV2 cells. COX2 may play a role in the activation of mouse microglia cells induced by manganese exposure.

关 键 词：锰 BV2细胞 环氧合酶2 

分 类 号：R-332[医药卫生]