降钙素基因相关肽对大鼠胃平滑肌的调控作用  

Regulation of calcitonin gene-related peptide on gastric smooth muscle in rats

作　　者：李聪[1] 蔡恒[2] 冯国营[2] 刘桂香[2] 许春胜[1] 

机构地区：[1]滨州医学院附属医院神经内科,滨州256603 [2]滨州医学院组织学与胚胎学教研室,烟台264003

出　　处：《解剖学杂志》2016年第2期145-148,共4页Chinese Journal of Anatomy

基　　金：山东省教育厅科技计划(J03K13);烟台市科技发展计划(2011073)

摘　　要：目的：探讨降钙素基因相关肽（CGRP）对大鼠胃平滑肌的调控作用。方法：贴块法原代培养大鼠胃平滑肌细胞，特异性平滑肌肌动蛋白a-actin免疫细胞化学鉴定。实验分CGRPI组（10-7mol／LCGRP）、CGRPⅡ组（10～mol／LCGRP）、CGRPI]]组（10-9^mol／LCGRP）和干预组（10-6mol／LCGRP8-37）与空白对照组。图像分析系统测定大鼠胃平滑肌细胞长度；Ca3＋荧光指示剂测定细胞内Ca2＋浓度；CellTiter-Glo试剂盒检测细胞内ATP含量。结果：CGRP可舒张胃平滑肌细胞，降低胃平滑肌细胞游离Ca2＋浓度及ATP含量。结论：CGRP通过受体介导直接参与胃平滑肌细胞松弛；CGRP可能通过降低胃平滑肌细胞游离Ca3＋浓度、ATP含量而发挥其调控作用。Objective： To investigate regulation of the calcitonin gene-related peptide （CGRP） on the gastric smooth muscle in rats. Methods： Primary rat gastric smooth muscle cells were cultured using tissue attachment block method. Specific smooth muscle a-actin antibody was stained by immunohistochemistry to identify the primary cultured cells. There were 5 groups： CGRP group I , Ⅱ ,Ⅲ （10-7 , 10-8 , 10-9 mol/L）group, CGRP 8-37 group（10-6 mol/L） and the control group. The length of smooth muscle cells was measured by micrometry before and after giving CGRP; by means of fluorescent indicator Fura-2/ AM, Ca2＋ were tested to show CGRP how to affect gastric smooth muscle cells; CellTiter-Glo Kit was used to detect intracellular ATP content. Results： Gastric smooth muscle cells of rats were isolated successfully. CGRP reduced gastric smooth muscle in various concentrations of free Ca2＋ concentration and ATP content. Conclusion： CGRP is directly involved in the relaxation of gastric smooth muscle cells by receptor mediator; CGRP plays a regulatory role in： gastric smooth muscle cells by reducing the concentration of free Ca2＋ and ATP content.

关 键 词：胃平滑肌细胞 降钙素基因相关肽 免疫组织化学 原代培养 

分 类 号：R57[医药卫生—消化系统]