腺苷预处理对氧糖剥夺/复氧糖诱导的星形胶质细胞及脂质运载蛋白-2表达的影响  

Effects of adenosine pretreatment on astrocytes and the expression of lipocalin-2 under oxygen-glucose deprivation and reoxygenation

作　　者：牛草原[1] 胡亚男[2] 王洁[1] 谭军[1] 

机构地区：[1]新乡医学院第三附属医院神经内科,新乡453000 [2]新乡医学院三全学院组织胚胎学教研究,新乡453000

出　　处：《解剖学杂志》2016年第2期192-195,共4页Chinese Journal of Anatomy

摘　　要：目的：探讨在氧糖剥夺／复氧糖（OGD／R）情况下腺苷预处理对体外培养星形胶质细胞的影响和脂质运载蛋白-2（LCN-2）表达的影响及其意义。方法：原代培养的大鼠星形胶质细胞随机分为腺苷预处理对照组（Ado—treated contr01）、对照组（Contr01）、实验组（AIX）／R）和模型组（OGD／R）。用MTT法检测各组细胞活力，ELISA检测各组OGD／R后细胞培养基中乳酸脱氢酶（LDH）的浓度，免疫荧光及免疫印迹检测各组LCN-2的相对表达。结果：腺苷预处理对照组与对照组的细胞活力、LDH浓度和LCN-2的表达量均无明显差别。与腺苷预处理对照组、对照组相比，模型组细胞损伤程度和LCN-2的表达量均明显升高。与模型组相比，实验组细胞损伤程度和LCN-2的表达量均明显降低。结论：腺苷可以通过减少LCN-2的释放明显改善OGD／R所引起的星形胶质细胞的损伤，从而增强脑组织对缺血再灌注损伤的耐受。Objective： To discuss the effect of adenosine pretreatment on astrocytes and lipocalin 2 （LCN-2） expression and its significance under oxygen-glucose deprivation and reoxygenation （OGD/R）. Methods： Primary cultured rat astrocytes were divided randomly into Ado-treated control group, control group, ADO/R group and OGD/R group. The cytoactivity was detected by MTT assay. The concentration of lactate dehydrogenase （LDH） in the cell medium was indicated by ELISA. The expression of LCN-2 was detected by immunofiuorescence and Western blotting. Results： The cytoactivity, the concentration of LDH and the expression of LCN-2 had no obvious difference between Ado-treated control and control group; The OGD/R group＇s cell damage degree and the LCN-2 expression increased significantly after the OGD/R compared with Ado-treated control and the control group; The ADO/R group＇s cell damage degree and the LCN-2 expression level decreased obviously compared with the OGD/R group. Conclusion： Adenosine preconditioning can decrease the ceil damage degree of astrocytes and increase the brain＇s tolerance to ischemia-reperfusion injury.

关 键 词：脂质运载蛋白-2 腺苷 星形胶质细胞 氧糖剥夺/复氧糖 

分 类 号：R741[医药卫生—神经病学与精神病学]