转运蛋白在脑出血模型中表达的实验研究  

Experimental study of translocator protein expression in an intracerebral hemorrhage model

作　　者：冯阳[1] 刘伟[1] 赵恒立[1] 戴志强[1] 刘昕[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科,全军神经外科研究所,全军神经创伤防治重点实验室,重庆400038

出　　处：《中华神经外科杂志》2016年第4期409-415,共7页Chinese Journal of Neurosurgery

基　　金：国家重点基础研究发展计划（973计划）（2014CB541600）

摘　　要：目的研究转运蛋白（TSPO）在小鼠脑出血模型中的表达情况。方法采用自体血注入法建立小鼠脑出血模型。应用28分法评价不同出血量的两组小鼠（平均出血量分别为15μl和25μl）在脑出血后1d的神经功能损伤情况，并采用Westernblot方法检测假手术组、出血量15μl组和出血量25出组的TSPO表达水平。以Westernblot方法及免疫荧光染色检测出血量25斗1组小鼠造模后12h，1、3及5dTSPO的表达情况。结果28分法评分结果显示，两组脑出血模型小鼠的神经功能评分较假手术组均有升高（均P〈0．05），且以出血量25μl组更为明显（P〈0．05）。Westernblot检测结果表明，两组脑出血模型小鼠TSPO的表达明显多于假手术组（均P〈0．05），且出血量25斗l组的TSPO表达高于出血量15μl组（P〈0．05）。在造模后12h内，出血量25μl组的TSPO表达水平与假手术组之间的差异无统计学意义（P〉0．05），造模后1、3及5dTSPO表达水平逐渐升高，且均高于假手术组（均P〈0．05）。免疫荧光染色结果表明，TSPO表达在造模后12h不明显，而在造模后1、3及5d逐渐增加，且多表达于血肿周围脑组织的小胶质细胞和星形胶质细胞。结论TSPO在小鼠脑出血后血肿周围脑组织中表达增多，其表达水平与出血后时间、出血量有关，提示可能与脑出血后继发性炎性反应及损伤相关。ObjectiVe To examine the expression o＇f translocator protein （TSPO） in an intracerebra] hemorrhage model in mice. Methods A intracerebral hemorrhage model in mice was induced by using autologous blood injection. The 28-point neurological deficit scale was used to evaluate neurological deficits of different amount of bleeding in the two group of mice （the mean amount of bleeding was 15 μl and 25 μl respectively） at day 1 after intracerebral hemorrhage, and Western blot was used to detect the expression levels of TSPO in the sham operation group, the amount of bleeding 15 μl group, and the amount of bleeding 25 μl group. Western blot and immunofluorescenee staining were used to detect the TSPO expression at 12 h, day 1, 3, and 5 after modeling in mice in the amount of bleeding 25 μl group. Results The results of the 28-poim neurological deficit scale showed that the neurological defect scores of the 2 groups of intracerebral hemorrhage model mice were significantly higher than the sham operation group （all P 〈0.05）, and the TSPO expression in the amount of bleeding 25 group was more significant higher than the amount of bleeding 15μl group （P 〈 0. 05）. Within 12 h after modeling, there was no significant difference in the TSPO expression between the amount of bleeding 25 pj group and the sham operation group （P 〉 0. 05）. The TSPO expression level increased gradually at day 1, 3, and 5 after modeling, and it was higher than the sham operation group （ all P 〈 0. 05 ）. Immunofluorescanee staining results showed that the TSPO expression was not obvious at 12 h after modeling, while it increased gradually at day 1,3, and 5 after modeling, and they mostly expressed in mieroglia and astrocytes of the perihematomal brain tissue. Cxmdusions The TSPO expression increases in perihematomal brain tissue after intracerebral hemorrhage in mice. Its expression level is associated with the time after bleeding and bleeding volume, suggesting that it may be associated with the secondary inflam

关 键 词：脑出血 转运蛋白 神经胶质增生 小鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]