微紫青霉菌次级代谢产物的化学成分和细胞毒活性  

Chemical constituents and their cytotoxic activities of the secondary metabolites of Penicillium janthinellum

作　　者：刘德胜[1] 黄玉玲[1] 马丽英[1] 卢昶军 刘为忠[1] 

机构地区：[1]滨州医学院药学院,山东烟台264003

出　　处：《中成药》2016年第4期830-834,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(31270082);山东省自然科学基金(ZR2013HM042);滨州医学院大学生科技创新活动基金资助(BY2013DKCX123)

摘　　要：目的研究微紫青霉菌Penicillium janthinellum次级代谢产物的化学成分和细胞毒活性。方法应用正相、反相硅胶、Sephadex LH-20凝胶、半制备HPLC色谱柱、重结晶等方法分离纯化真菌发酵液的次级代谢产物,质谱、核磁共振解析所得化合物的结构,MTT法测试其对人肺腺癌细胞A549的细胞毒活性。结果从中分离得到8个化合物,分别鉴定为chrodrimanin B（1）、striatisporolide A（2）、methylenolactocin（3）、cyclo（Leu-Tyr）（4）、cylco（Phe-Tyr）（5）、cylco（Phe-Val）（6）、cyclo（Tyr-Pro）（7）、麦角甾醇（8）。结论所有化合物均为首次从该真菌中分离得到,其中化合物1～3具有较弱的抗A549细胞毒活性。AIM To study the chemical constituents and their cytotoxic activities of the secondary metabolites of Penicillium janthinellum. METHODS The secondary metabolites of fungus fermentation broth were isolated and purified by silica,ODS,Sephadex LH-20 gel,semi-preparative HPLC column and recrystallization,the structures of obtained compounds were identified by MS and NMR. Then their cytotoxic activities against human lung adenocarcinoma cell A549 were tested by MTT method. RESULTS Eight compounds were isolated and identified as chrodrimanin B（ 1）,striatisporolide A（ 2）,methylenolactocin（ 3）,cyclo（ Leu-Tyr）（ 4）,cylco（ PheTyr）（ 5）,cylco（ Phe-Val）（ 6）,cyclo（ Tyr-Pro）（ 7）,ergosterol（ 8）. CONCLUSION All compounds are isolated from this fungus for the first time. Among them,compounds 1- 3 exhibit weak cytotoxic activities against A549.

关 键 词：微紫青霉菌 次级代谢产物 化学成分 细胞毒活性 人肺腺癌细胞A549 MTT法 

分 类 号：R284.1[医药卫生—中药学]