鸡蛋过敏人群中主要过敏原的反应性及卵类粘蛋白抗原表位的初步研究  

Effect of major allergen in egg allergy and preliminany analysis of ovomuciod epitopes in egg-allergy childrens

作　　者：楚海荣[1] 林书祥[2] 侯丽英[3] 张盈莹 朱黎娜 闫娟娟[3] 李会强[3] 

机构地区：[1]潍坊医学院医学检验学系,山东潍坊261053 [2]天津市儿童医院,天津300204 [3]天津医科大学医学检验学院,天津300203

出　　处：《中国食品卫生杂志》2016年第2期144-148,共5页Chinese Journal of Food Hygiene

基　　金：国家自然基金项目(81371882);山东省高等学校优秀青年教师国内访问学者项目

摘　　要：目的分析年龄、性别对鸡蛋过敏人群中主要过敏原反应性的影响,初步探索我国鸡蛋过敏儿童卵类粘蛋白的主要抗原表位。方法以卵类粘蛋白、卵白蛋白、卵转铁蛋白纯品为抗原,采用免疫斑点印迹,检测21例鸡蛋过敏儿童血清特异性免疫球蛋白E（SIgE）与蛋白反应性,分析年龄、性别因素对过敏原反应性的影响;应用DNAStar生物分析软件,预测并合成卵类粘蛋白抗原表位肽段,采用免疫斑点印迹检测抗原表位与鸡蛋过敏儿童血清的反应性。结果 A（≤1岁）组、B组（2～3岁包括3岁）、C组（〉3岁）的卵类粘蛋白反应率差异有统计学意义（P〈0.05）,卵白蛋白、卵转铁蛋白反应率各组间均差异无统计学意义（P〉0.05）;性别对各过敏原的反应率差异无统计学意义（P〉0.05）;卵类粘蛋白抗原表位反应率较低。结论卵类粘蛋白sIgE在不同年龄段存在差别,卵白蛋白sIgE在21例患者均可检测出,卵转铁蛋白s Ig E在各年龄段无明显差别;应用DNAStar生物软件预测的卵类粘蛋白抗原表位,在天津地区反应性低,不是该地区主要抗原表位。Objective To analyze the effectof age and gender on egg allergens,and to explore the main epitopes of OM in chinese population.Methods 21 patients diagnosed as egg allergy were included,patients were divided into 3 groups：younger than 1-year-old（group A,6 subjects）,2 to 3-years-old（group B,7 subjects）,and elder than 3-years-old（group C,8 subjects）.The serum of patients,the specific Ig E of ovomuciod,ovalbumin,ovotransferrin and epitopes of ovomuciod were detected by immunodot-blots.Epitopes of ovomuciod were predicted by DNAStar.Results Immunodot-blots analysis showed that the numbers of serum samples which could react with ovomuciod were 2 in group A,7 in group B and 5 in group C,respectively.All patients samples showed positive to ovalbumin.3 patients Ig E in group A could binding to ovotransferrin,as well as 6 samples in group B and 5 samples in group C.Conclusion Ovomuciod s Ig E reactivity to egg allergens differed according to children＇s age.The DNAStar biological software was used to predict the epitopes of the egg type mucin which was low in Tianyin.

关 键 词：鸡蛋过敏 特异性免疫球蛋白E 卵类粘蛋白 抗原表位 食物过敏 食品 儿童 

分 类 号：R725.9[医药卫生—儿科] R446.6[医药卫生—临床医学]