AMH-T体外抗肿瘤作用的研究  

In Vitro Anticancer Effect of AMH-T

作　　者：王静[1] 贺小琼[1] 

机构地区：[1]昆明医科大学公共卫生学院,云南昆明650500

出　　处：《昆明医科大学学报》2016年第4期4-7,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(30960437);云南省科技计划项目(2009FXL001);云南省科技厅-昆明医科大学联合专项基金重点资助项目(2012FB001)

摘　　要：目的探讨功效部位AMH-T对人Hela、Hep G2和U251体外抗肿瘤作用,了解其剂量-反应和时间-效应关系,发现对AMH-T敏感的肿瘤细胞株.方法采用体外抗肿瘤实验进行体外抗癌活性检测.实验设阴性对照组(DMSO)、阳性对照组(DDP,3μM)、AMH-T的5个剂量组(2、4、8、16和32μg/m L).采用MTT法检测药物处理24 h、48 h和72 h后的OD值,计算各组的增殖抑制率.结果 AMH-T作用72 h后,在AMH-T为8μg/m L时,显微镜下观察Hela细胞几乎死亡.AMH-T在剂量为2、4、8、16和32μg/m L时,对人宫颈癌细胞株Hela的抑制率分别为-5.54%、2.93%、72.14%、74.41%和73.86%,其IC50为11.66μg;对人肝癌细胞株Hep G2的抑制率分别为3.87%、12.50%、54.07%、55.27%和51.86%,其IC50为22.90μg;对人神经胶质瘤细胞株U251的抑制率分别为-5.47%、1.08%、54.83%、57.75%和71.78%,其IC50为16.85μg.结论 AMH-T对Hela、Hep G2和U251均具有显著性的体外抗肿瘤作用,存在明显的剂量-反应和时间-效应关系;Hela细胞株对AMH-T最为敏感.Objective To investigate the in vitro anticancer effects of the bioactive fraction AMH- T on human cancer cell lines Hela,U251,Hep G2 and the dose- effect and time- effect relationship. Me thods MTT assay was used to evaluate the anticancer effect of AMH- T in vitro. The experiment set the negative group（DMSO）, the positive group（DDP, 3 μM）, five dosage groups of AMH- T（2, 4, 8, 16, 32 μg/m L）. The tumor inhibition rate in each group was tested by MTT assay on 24 h, 48 h and 72 h after treatment. Re s ults After 72 h treatment with 8 μg/ml of AMH- T,most Hela cells died under inverted microscope. The inhibitory ratios of AMH- T at 2, 4, 8, 16, 32 μg/m L on Hela cells were- 5.54%, 2.93%, 72.14%, 74.41% and 73.86%; 3.87%,12.50%, 54.07%, 55.27% and 51.86% on Hep G2 cells, and- 5.47%, 1.08%, 54.83%, 57.75% and71.78% on U251 cells. The IC50 values against Hela, Hep G2 and U251 were 11.66 μg, 22.90 μg, and 16.85μg, respectively. Conclus ions AMH- T has significant anticancer effects on Hela, Hep G2 and U251 cells in a concentration and time dependent manner. Hela cell line is most sensitive to AMH- T among three cell lines.

关 键 词：天然产物 宫劲癌 肝癌 神经胶质瘤 体外抗癌 MTT法 

分 类 号：R730[医药卫生—肿瘤]