胰岛素对脑梗死小鼠梗死周围区血管直径和梗死体积的影响  

Effects of insulin on vascular diameter of the peri -infarct region and infarct volume after cerebral infarction in mice

作　　者：吴腾腾[1] 李明月[1] 邝亚晗 裴中[1] 陶玉倩[1] 

机构地区：[1]中山大学附属第一医院神经内科,广州510080

出　　处：《国际脑血管病杂志》2016年第2期174-179,共6页International Journal of Cerebrovascular Diseases

基　　金：广东省科技计划（201213031800106;201313051000018）

摘　　要：目的：探讨胰岛素对脑梗死小鼠梗死周围区血管直径和脑梗死体积的影响。方法40只雄性 C57/BL6j 小鼠随机分为正常对照组（n =5）、脑梗死组（n =15）、脑胰岛素抵抗组（n =5）和脑胰岛素抵抗梗死组（n =15）。采用光化学法制作脑梗死模型，侧脑室注射链脲霉素建立脑胰岛素抵抗模型，双光子共聚焦显微镜在体评价小脑延髓池注射胰岛素20 min后梗死周围区血管直径的改变。脑梗死模型制作后立即经小脑延髓池给予人工脑脊液或胰岛素（10 ng/ml），并于梗死后24 h评估胰岛素对脑梗死体积的影响。结果胰岛素对正常对照组小鼠各类脑血管直径均无显著影响，但能显著收缩脑胰岛素抵抗组和脑胰岛素抵抗梗死组梗死周围区脑动脉[分别为（－23.16±6.86）％和（－23.32±6.40）％； P 均〈0.001]和穿支动脉[分别为（－15.20±5.51）％和（－16.40±4.27）％；P 均〈0.001]，对脑静脉直径无显著影响。脑梗死组与正常对照组以及脑胰岛素抵抗梗死组与脑胰岛素抵抗组胰岛素的血管活性效应均无显著差异。胰岛素可使脑梗死组脑梗死体积显著缩小[（9.0±1.0）mm3对（6.0±1.2）mm3；t =4.294，P =0.002]。而胰岛素治疗对胰岛素抵抗脑梗死组脑梗死体积无显著影响[（12.6±2.3）mm3对（11.6±1.7）mm3；t =0.782，P =0.456]。结论胰岛素能减轻正常小鼠缺血性脑损伤且不影响梗死周围区脑血管直径，但对脑胰岛素抵抗小鼠的神经保护作用并不明显，可能与其对梗死周围区的缩血管效应有关。Objective To investigate the effects of insulin on vascular diameter of the peri -infarct region and infarct volume after cerebral infarction in mice. Methods Forty male C57/BL6j mice w ere randomly divided into a control group （ n = 5）, a cerebral infarction group （ n = 15）, a cerebral insulin resistance group （n = 5）, and a cerebral insulin resistance infarction group （ n = 15）. A model of cerebral infarction w as induced by the photochemical method. A model of cerebral insulin resistance w as induced by intracerebroventricular injection of streptozocin. Tw o -photon confocal microscope w as used to in vivo evaluate the changes of vascular diameter in the peri-infarct region at 20 min after insulin injection into the cerebelomedulary cistern. After modeling of cerebral infarction, artificial cerebrospinal fluid or insulin （10 ng/ml） w as immediately injected into the cerebelomedulary cistern, and the effect of insulin on cerebral infarct volume w as evaluated at 24 h after infarction. Results Insulin did not have significant effect on various types of cerebral vascular diameters in the normal control group, but it significantly contracted cerebral arteries （ -23.16% ±6.86% and -23.32% ±6.40%, respectively; al P 〈0.001） and penetrating arteries （ -15.20% ±5.51% and -16.40% ±4.27%, respectively; al P 〈 0.001） in the cerebral insulin resistance group and the cerebral insulin resistance infarction group, but it did not have any effect on the diameters of the cerebral veins. There w ere no significant differences in the vasoactive effects of insulin betw een the cerebral infarction group and the normal control group, as w el as betw een the cerebral insulin resistance group and the cerebral insulin resistance infarction group. Insulin significantly reduced the volumenbsp;of cerebral infarction in the cerebral infarction group （9.0 ±1.0 mm3 vs.6.0 ±1.2 mm3; t = 4.294,P =0.002）, and it did not have significant effect on the volume of cerebral infarction in the cerebral insulin

关 键 词：脑梗死 脑缺血 胰岛素 胰岛素抗药性 微循环 血管舒张 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]