基质细胞衍生因子1促进骨髓干细胞增殖:200μg/L为最佳质量浓度  

Stromal cell derived factor-1 promotes the proliferation of bone marrow stem cells:the optimal concentration is 200μg/L

作　　者：彭伟[1] 秦媛[2] 廖春晖 陈松龄[4] 

机构地区：[1]中山大学附属第一医院口腔科,广东省广州市510080 [2]第四军医大学附属口腔医院急诊与综合临床科,陕西省西安市710032 [3]广州市妇女儿童医疗中心口腔科,广东省广州市510623 [4]中山大学附属第一医院口腔颌面外科,广东省广州市510080

出　　处：《中国组织工程研究》2016年第10期1402-1408,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81371111);广东省自然科学基金(2014A030313059)~~

摘　　要：背景:基质细胞衍生因子1是一类对免疫细胞有趋化作用的小分子蛋白,具有广泛的生物学活性,对骨髓干细胞和牙周膜细胞都有趋化作用。目的:观察基质细胞衍生因子1对比格犬骨髓干细胞增殖的影响并探讨最佳质量浓度。方法:利用100,200,300μg/L基质细胞衍生因子1对比格犬骨髓干细胞进行干预,MTT比色实验检测骨髓干细胞的增殖情况,筛选出最佳质量浓度,再次采用MTT比色实验检测最佳质量浓度干预后骨髓干细胞的增殖情况。结果与结论:基质细胞衍生因子1在100,200,300μg/L时对骨髓干细胞均有促进作用,但200μg/L与300μg/L效果更为显著,且这两者差异无显著性意义,因此选择200μg/L作为最佳质量浓度。相对于空白对照组来说,200μg/L最佳质量浓度组细胞增殖明显。结果表明基质细胞衍生因子1能促进骨髓干细胞的增殖,且质量浓度为200μg/L时促增殖作用最显著。BACKGROUND： Stromal cell derived factor-1 is a small molecular protein with a wide range of biological activity that can cause immune cell chemotaxis, and it also has a chemotactic effect on bone marrow stem cells and periodontal ligament cells. OBJECTIVE： To investigate the effect of stromal cell derived factor-1 with different concentrations on the proliferation of bone marrow stem cells and to probe the best concentration. METHODS： Bone marrow stem cells from beagle dogs were cultured in vitro and stimulated by different concentrations of stromal cell derived factor-1（100, 200, 300 μg/L）. MTT was used to detect the influence of stromal cell derived factor-1 on the proliferation of bone marrow stem cells so as to screen the best concentration of stromal cell derived factor-1. Then, stromal cell derived factor-1 at the best concentrations was used to intervene the bone marrow stem cells, and MTT was used again to detect the proliferation of bone marrow stem cells. RESULTS AND CONCLUSION： Stromal cell derived factor-1 at concentrations of 100, 200, 300 μg/L could promote the proliferation of bone marrow stem cells, and the effect was more notable at 200 and 300 μg/L but with no significant difference. Therefore, 200 μg/L was considered to be the best concentration of stromal cell derived factor-1 for intervention of bone marrow stem cells. Compared with the blank control group, 200 μg/L stromal cell derived factor-1 could significantly promote the proliferation of bone marrow stem cells. Taken together, stromal cell derived factor-1 can promote the proliferation of bone marrow stem cells, and its best concentration is 200 μg/L.

关 键 词：干细胞 骨髓干细胞 基质细胞衍生因子1 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]