异氟醚抑制海马神经干细胞增殖并可促其向神经元分化  

Isoflurane inhibits neural stem cell proliferation in the hippocampus and promotes its differentiation into neurons

作　　者：孙鼐[1] 李春伟 赵伟新[1] 宋琼[1] 夏贵山[1] 

机构地区：[1]郑州大学附属郑州中心医院麻醉科,河南省郑州市450007 [2]西平县人民医院麻醉科,河南省周口市466600

出　　处：《中国组织工程研究》2016年第10期1488-1493,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省医学科学研究重点课题计划(20120088)~~

摘　　要：背景:异氟醚不仅可诱发新生大鼠广泛的大脑神经元凋亡,还可抑制新生大鼠海马神经发生,引起海马依赖性的学习记忆缺陷。目的:探讨异氟醚对海马神经干细胞增殖、分化的影响。方法:26只SD大鼠随机分为空气组和异氟醚组,每组13只。异氟醚组大鼠先吸入体积分数2.5%异氟醚3 min后再吸入体积分数1.5%异氟醚维持4 h,空气组仅吸入空气。干预后检测两组大鼠血糖、动脉血气指标;干预前以及干预结束后腹腔注射5-溴脱氧尿苷,末次注射5-溴脱氧尿苷后24 h取脑组织,制备冰冻切片,进行免疫荧光染色。结果与结论:(1)两组大鼠pH、PaO_2、PaC O_2、HCO_3、BE以及SaO_2水平差异无显著性意义(P>0.05)。(2)异氟醚组大鼠BrdU^+细胞计数显著少于空气组(P<0.05)。(3)异氟醚组NeuroD^+/BrdU^+细胞显著高于空气组(P<0.05)。(4)异氟醚组不良反应发生率为23%,显著高于空气组(7.7%),差异有显著性意义(P<0.05)。(5)结果表明异氟醚能够抑制海马齿状回神经干细胞增殖并促进其向神经元分化。BACKGROUND： Isoflurane cannot only induce a wide range of large neuronal apoptosis, but also inhibit hippocampal neurogenesis in neonatal rats, thereby resulting in hippocampus-dependent learning and memory defects. OBJECTIVE： To investigate the isoflurane effect on proliferation and differentiation of the hippocampal neural stem cells. METHODS： Twenty-six Sprague-Dawley rats were randomly divided into air group and isoflurane group（n=13 per group）. Rats in the isoflurane group were subjected to 2.5% isoflurane inhalation for 3 minutes followed by 1.5% isoflurane inhalation for 4 hours. Rats in the air group only breathed in air. After the intervention, blood glucose and arterial blood gas changes were detected in the two groups. Additionally, rats in the two groups were given intraperitoneal injection of 5-bromodeoxyuridine before and after intervention. At 24 hours after the last injection of 5-bromodeoxyuridine, brain tissues were taken to make frozen sections for immunofluorescence staining. RESULTS AND CONCLUSION： There were no significant difference in pH, PaO2, PaCO2, HCO3, BE and SaO2 levels between the two groups（P〈0.05）. Compared with the air group, the number of BrdU^＋ cells was significantly less in the isoflurane group（P〈0.05）, while the number of NeuroD^＋/BrdU^＋ cells was significantly higher in the isoflurane group（P〈0.05）. The incidence of adverse reactions was 23% in the isoflurane group, which was significantly higher than that in the air group（7.7%; P〈0.05）. These findings indicate that isoflurane can inhibit the proliferation of neural stem cells in the hippocampal dentate gyrus, and promote their differentiation into neurons.

关 键 词：干细胞 分化 神经干细胞 异氟醚 海马 细胞增殖 细胞分化 不良反应 

分 类 号：R394.2[医药卫生—医学遗传学]