骨髓间充质干细胞移植改善慢性哮喘气道炎症  

Bone marrow mesenchymal stem cell transplantation improves airway inflammation due to chronic asthma

作　　者：张起[1] 郭蕊蕊[1] 胡江平[1] 

机构地区：[1]郑州大学附属郑州中心医院呼吸内科,河南省郑州市450000

出　　处：《中国组织工程研究》2016年第10期1494-1500,共7页Chinese Journal of Tissue Engineering Research

基　　金：2014年度河北省医学科学研究重点课题计划(ZD20140271)~~

摘　　要：背景：以往研究提示骨髓间充质干细胞移植能够通过减轻炎症程度来改善病情。目的：探讨骨髓间充质干细胞对慢性哮喘大鼠的治疗效果。方法：通过卵白蛋白腹腔注射加雾化刺激的方式制备大鼠慢性哮喘模型,待模型稳定后,尾静脉注射4×10^5及8×10^5个骨髓间充质干细胞。治疗30 d后,苏木精-伊红染色观察肺组织病理变化,RT-qPCR、ELISA法检测肺组织和外周血中白细胞介素10、肿瘤坏死因子α、γ-干扰素水平。结果与结论：（1）卵白蛋白腹腔注射配合雾化刺激的方法可以制备稳定的慢性哮喘模型。（2）治疗后30 d,肺组织病理显著改善,肺组织及外周血中炎症因子白细胞介素10、肿瘤坏死因子α、γ-干扰素水平显著下降,两治疗组之间的数据差异无显著性意义。（3）实验结果表明骨髓间充质干细胞具有治疗慢性哮喘的潜力。BACKGROUND： Studies have shown that bone marrow mesenchymal stem cell transplantation can improve disease conditions by reducing inflammation. OBJECTIVE： To explore the therapeutic efficacy of bone marrow mesenchymal stem cells on chronic asthma rats. METHODS： A rat model of chronic asthma was established by intraperitoneally injected and aerosolized ovalbumin. After modeling, rats were given 4×10^5 and 8×10^5 bone marrow mesenchymal stem cells via the tail vein, respectively. Thirty days later, the lung tissues were observed pathologically using hematoxylin-eosin staining; RT-q PCR and ELISA methods were employed to test the changes in interleukin-10, tumor necrosis factor-α and interferon-γ levels in lung tissue and peripheral blood, respectively. RESULTS AND CONCLUSION： Rat models of chronic asthma were successfully established after intraperitoneal injection of ovalbumin combined with aerosolized ovalbumin. After 30 days of cell treatment, the structure of lung tissues were obviously recovered, and the levels of interleukin-10, tumor necrosis factor-α and interferon-γ showed some improvement in lung tissue and peripheral blood, but there were no differences between the two groups. In conclusion, bone marrow mesenchymal stem cells show some potential role in the treatment of chronic asthma.

关 键 词：干细胞 移植 慢性哮喘 骨髓间充质干细胞 气道重塑 免疫平衡 干细胞移植 支气管炎症 

分 类 号：R394.2[医药卫生—医学遗传学]