长链非编码RNA LINC01126异常高表达促进前列腺癌发生发展  

Ectopic Expression of Long Non-Coding RNA LINC01126 Affects Prostate Development

作　　者：浦洪雷 万学超 张溥[1] 王丹[1] 吴海[1] 李瑶[1] 

机构地区：[1]复旦大学生命科学学院,上海市工业菌株工程技术研究中心,上海200438

出　　处：《中国科学：生命科学》2016年第4期432-440,共9页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:31571330);上海科学技术委员会基地项目(批准号:13DZ2252000)资助

摘　　要：长链非编码RNA(lncRNA)在疾病的发生发展过程中发挥着重要的作用.LncRNA的异常表达与许多肿瘤的发生发展密切相关.本研究通过分析肿瘤基因图集(TCGA)数据库首次鉴定出了在前列腺癌中高表达的长链非编码RNA LINC01126,并在前列腺癌样本和前列腺癌细胞系中得到了验证.通过进一步分析发现,LINC01126的表达量在肿瘤中显著上调(419例前列腺癌组织样本和52例癌旁组织样本,P<0.001),LINC01126表达与临床病理分级的相关性分析中,相比于癌旁组织,Gleason 8样本和Gleason 9样本中的LINC01126表达水平显著增加(52例癌旁组织样本;Gleason 8,n=44,P<0.001;Gleason 9,n=71,P<0.001).进一步的分析结果表明,LINC01126能够较好地指示前列腺癌的Gleason分级.相比于Gleason 6样本,Gleason 8样本和Gleason 9样本中的LINC01126表达水平上调(Gleason 6,n=30;Gleason 8,n=44,P<0.01;Gleason 9,n=71,P<0.05).同样,相比于Gleason 7样本,Gleason 8样本和Gleason 9样本中的LINC01126表达水平也显著增加(Gleason 7,n=193;Gleason 8,n=44,P<0.001;Gleason 9,n=71,P<0.001).LINC01126的表达水平与前列腺癌病理分期的相关性结果提示,LINC01126在发生转移的前列腺癌中的表达量更高,差异显著(T2a,T2b,T2c样本共144例,T3a,T3b,T4样本共194例,P<0.01).另外,还分析了与LINC01126共表达或相反表达的mRNA,作为其可能的调控基因,及其这些基因的生物学进程和相关的信号通路,表明LINC01126与前列腺癌发生发展具有潜在的生物学关联,有望成为前列腺癌的生物标志物.Long non-coding RNA（lncRNA） plays an important role in the development of various diseases. Abnormal expression of lncRNAs is closely associated with tumorigenesis. In this study, we identified a long non-coding RNA, LINC01126, which was over-expressed in prostate cancer. LINC01126 was found to be up-regulated in prostate cancer（419 samples, 52 controls, P〈0.001） by analyzing the Cancer Genome Atlas database. Compared with that in normal tissues, LINC01126 expression was up-regulated in Gleason 8 and Gleason 9 samples（52 control; Gleason 8, n = 44, P〈0.001; Gleason 9, n = 71, P〈0.001）. Further analysis showed that LINC01126 was a significant indicator of prostate Gleason classification. LINC01126 expression increased in Gleason 8 and 9 samples compared with that in Gleason 6（Gleason 6, n = 30; Gleason 8, n = 44, P〈0.01; Gleason 9, n = 71, P〈0.05） and Gleason 7 samples（Gleason 7, n = 193; Gleason 8, n = 44, P〈0.001; Gleason 9, n = 71, P〈0.001）. Additionally, our results showed that LINC01126 was highly expressed in metastatic prostate cancer（144 T2a, T2b, and T2c samples, 194 T3a, T3b, and T4 samples, P〈0.01）. We also identified genes that were negatively or positively regulated by LINC01126 as well as their roles in biological processes and related pathways. In conclusion, our results suggest that LINC01126 is a new molecular biomarker for the diagnosis of prostate cancer.

关 键 词：长链非编码RNA LINC01126 前列腺癌 生物标志物 

分 类 号：R737.25[医药卫生—肿瘤]