不同侵袭转移特性肝癌模型的 ^(18)F-FDG和2- ^(18)F-乙酸盐联合显像  

Binding ^(18)F-FDG and ^(18)F-fluoroacetate Micro-PET imaging in human HCC model

作　　者：何婷婷[1] 王卉[1] 徐白萱[2] 刘健[2] 张小军[2] 田嘉禾[2] 

机构地区：[1]解放军总医院海南分院,海南三亚572013 [2]解放军总医院,北京100853

出　　处：《中国临床医学影像杂志》2016年第4期267-270,共4页Journal of China Clinic Medical Imaging

基　　金：三亚市医疗卫生创新项目(2015YW27)

摘　　要：目的:探讨^(18)F-FDG和2-^(18)F-乙酸盐联合显像在区分不同转移潜能的肝癌裸鼠模型中的意义。方法:以人肝癌细胞株Hep G2和QGY7701为研究对象进行体外侵袭实验,判断细胞侵袭转移能力的大小。通过体外核素摄取实验观察两株肝癌细胞^(18)F-FDG和2-^(18)F-乙酸盐的摄取差异性,并建立皮下荷瘤模型进一步观察两种示踪剂的活体显像特征,绘制感兴趣区(ROI)并计算瘤/非瘤比值。结果:Hep G2细胞体外侵袭能力大于QGY7701(P<0.05)。Hep G2细胞的^(18)F-FDG和2-^(18)F-乙酸盐摄取率均高于QGY7701,其摄取差异有统计学意义(P<0.05)。两株细胞^(18)F-FDG摄取率与侵袭能力呈正相关(r=0.657,P<0.05)。活体显像中Hep G2肿瘤组织的^(18)F-FDG和2-^(18)F-乙酸盐的摄取值亦高于QGY7701,其中^(18)F-FDG的摄取差异有统计学意义(P<0.05)。结论:^(18)F-FDG摄取值能够反映肝癌细胞侵袭转移能力的大小;同时^(18)F-FDG Micro-PET显像能够区分两种肝癌模型,提示^(18)FFDG显像有助于预测肝癌的不同侵袭转移潜能力。Objective： The aim of study was to evaluate whether the ^（18）F-FDG and ^（18）F-fluoroacetate（ ^（18）F-FAC） could be used to distinguish the different metastatic potential of hepatocellular carcinoma（HCC） models. Methods： Human HCC cell lines Hep G2 and QGY7701 were cultured and implanted into nude mice to create HCC models. In vitro cell invasion assay was performed to judge invasion ability. Uptakes of ^（18）F-FDG, ^（18）F-FAC in two cells were assessed by in vitro uptake assay. In vivo imaging of tumor-bearing mice were obtained by animal PET/CT after injection of each tracer. ROI（region of interest） was drawn over tumor and lung to calculate T/NT（tumor/non-tumor）. Results： The invasion ability of Hep G2 was much higher than QGY7701（P〈0.05）, and the uptake ratio of ^（18）F-FDG and ^（18）F-FAC in Hep G2 was higher than that in QGY7701（P〈0.05） in vitro uptake assay. The ^（18）F-FDG uptake of cells correlated with invasion capability（r=0.657, P〈0.05）. Meanwhile, in Micro-PET/CT study, the uptake of ^（18）F-FDG and ^（18）F-FAC in Hep G2 tumor was higher than that in QGY7701 tumor（P〈0.05）. Conclusion： High uptake of ^（18）F-FDG reflects higher invasion ability of HCC cell. Meanwhile, there is differentiation between two HCC models in ^（18）F-FDG Micro-PET imaging. It indicates that ^（18）F-FDG PET imaging could help to predict metastasis ability of HCC.

关 键 词：肝肿瘤 氟脱氧葡萄糖F18 乙酸盐类 正电子发射断层显像术 

分 类 号：R735.7[医药卫生—肿瘤] R817.4[医药卫生—临床医学]