As_2O_3对胃癌细胞中肿瘤干性相关因子表达的抑制作用  

As_2O_3 inhibits the expression of stem factors in gastric cancer cells

作　　者：张琳[1] 杨家佳[1] 徐玉婷[1] 柳红[1] 吴永平[1] 王庆苓[1] 

机构地区：[1]徐州医学院病理学教研室,徐州221004

出　　处：《临床与实验病理学杂志》2016年第4期375-379,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：江苏省高校自然科学基金(12KJD320006)

摘　　要：目的观察As_2O_3对胃癌干性相关因子CD44、EpCAM表达的影响,并探讨其可能的作用机制。方法体外实验:以MGC803细胞株为实验对象,给予4μmol/L的As_2O_3处理48 h后,Western blot及RT-PCR法检测CD44、EpCAM的表达。体内实验:建立人胃癌MGC803细胞裸鼠移植瘤模型,分为对照组和As_2O_3(5 mg/kg/天)组,观察裸鼠体内肿瘤的形成及瘤体组织的体积和重量;给药10天后处死裸鼠,分别用Western blot和RT-PCR法检测两组肿瘤中CD44、EpCAM的表达。结果体外:细胞在用As_2O_3处理后,与对照组相比,As_2O_3组中CD44、Ep CAM的蛋白和mRNA表达均减少(P<0.05)。体内:与对照组相比,As_2O_3组中裸鼠的移植瘤体积和重量明显降低;Western blot和RT-PCR检测结果均表明在As_2O_3组中移植瘤的CD44、EpCAM的蛋白和mRNA表达均降低,差异有统计学意义(P<0.05)。结论 As_2O_3在体内和体外水平均抑制了CD44、EpCAM的表达,因此As_2O_3对胃癌中的肿瘤干细胞种群具有明显的抑制作用。Purpose To observe the effect of As2O3 on expression of stem factors CD44, EpCAM of gastric cancer and to explore the mechanism. Methods In vitro, human gastric cancer MGC803 cells were cultivated and treated with As2O3 （4 μmoL/L） for 48 h. The expression of CD44, EpCAM was detected by Western blot and RT-PCR respectively. In vivo, the MGC803 cells were cultivated and inoculated into the subcutaneous tissue of the left flank of Balb/c mice. The transplantation tumor model of human gastric cancer in mice was successfully established. The mice were randomly divided into two groups： control group and As2O3 （5 mg/kg/day） group. The tumor size and weight were measured, and the tumor inhibitory rate was calculated. The mice were treated for ten days, and then they were samificed after 24 hours of the last treatment. The expression of CD44 and EpCAM was identified by Western blot and RT- PCR. Results In vitro study showed that protein and mRNA expression of CD44 and EpCAM in MGC803 cells treated with As2O3 was lower than control group （ P 〈 0.05 ）. In vivo showed that the tumor weight was lower and the tumor size was smaller in As2O3 group as compared with control group. The expression of CD44 and EpCAM in As2O3 group was decreased （ P 〈 0. 05 ）. Conclusion As2O3 could obviously inhibit the expression of CD44 and EpCAM in vitro and in vivo. As2O3 may play an important role in inhibiting gastric cancer stem cells

关 键 词：胃肿瘤 AS2O3 CD44 EPCAM 

分 类 号：R735.2[医药卫生—肿瘤]