高压脉冲电场法联合提取鹿茸中蛋白质、多糖的工艺优化  

Optimization of Extration Process of Protein and Polysaccharides from Antler Velvet Assisted by High Intensity Pulsed Electric Fields

作　　者：董思敏 王海璐[1] 王全凯[1] 殷涌光[3] 张晶[1,2] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林省中韩动物科学研究院,长春130600 [3]吉林大学,长春130022

出　　处：《中国药学杂志》2016年第8期620-624,共5页Chinese Pharmaceutical Journal

基　　金：吉林省科技发展计划资助项目(20140204063YY;20130413044GH;20130302012NY)

摘　　要：目的采用高压脉冲电场技术从鹿茸中同步提取蛋白质和多糖类成分,并对提取工艺进行优化。方法以水为溶剂,对电场强度、脉冲数、料液比3个因素进行考察,通过正交实验确定高压脉冲电场技术联合提取鹿茸中蛋白质和多糖的最优工艺。结果按料液比1∶30 g·m L-1、电场强度20 k V·cm-1、脉冲数为10的条件下进行高压脉冲电场提取,蛋白质提取率达到12.32%、多糖提取率可达3.25%。结论该工艺可有效地从鹿茸中联合提取水溶性蛋白和多糖。OBJECTIVE To optimize the extraction proces of protein and polysaccharides from Antler Velvet using high intensity pulsed electric fields. METHODS With water as solvent,electric field intensity,pulse number and solid-liquid ratio as investigation parameters,an L9（ 3^4） orthogonal test was used to determine the optimal process for simultaneous extraction of protein and polysaccharides from Antler Velvet. RESULTS When the extraction by high intensity pulsed electric fields was conducted at the solid-liquid ratio of 1 ∶ 30 g · mL- 1and electric field intensity of 20 kV · cm^- 1,with pulse number of 10,the protein extraction rate reached12. 32%,and polysaccharides extraction rate could reach 3. 25%. CONCLUSION The optimized process can effectively and simutaneously extract protein and polysaccharides from Antler Velvet.

关 键 词：高压脉冲电场 鹿茸 蛋白质 多糖 工艺优化 

分 类 号：R282[医药卫生—中药学]