颞下颌关节盘前移位后VEGF-C基因在髁突软骨中的表达及意义  

The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance

作　　者：徐高丽 汪淑华[1] 史秋涛 柳毅[1] 阎帆[1] 徐婷[2] 谷志远[1] 

机构地区：[1]浙江中医药大学口腔医学院,浙江杭州310053 [2]浙江大学医学院附属第一医院口腔科,浙江杭州310003

出　　处：《上海口腔医学》2016年第2期136-140,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81170979;81400533);浙江省医药卫生科技计划项目(2015KYB146)

摘　　要：目的:探讨血管内皮细胞生长因子C在颞下颌关节盘前移位后髁突软骨中的表达及意义。方法:采用日本大耳白兔60只,48只用弹力丝牵拉关节盘前移,建立关节盘前移模型,分别于术后1、2、4、8周处死12只,12只空白对照组同期处死,取出颞下颌关节标本。利用基因芯片技术进行筛选,实时荧光定量PCR进行验证和SP免疫组化技术检测不同组内髁突软骨中VGEF-C的表达与分布。采用SPSS19.0软件包对每组样本实验组和对照组数据进行配对t检验。结果:颞下颌关节盘前移位后,在基因水平上VEGF-C相比于正常对照组均有不同程度变化,早期VEGFC表达减少,第2周时降至最低水平,差异显著(P<0.05);第8周时,VEGF-C表达有所增加。镜下观察可见VEGF-C在髁突软骨增殖层、肥大层及钙化软骨层中均有表达,1周组阳性表达主要见于增殖深层和肥大层,钙化软骨层也有弱阳性表达。2周组和4周组阳性表达主要位于肥大层,且在钙化软骨层中表达逐渐增强。8周组肥大层有阳性表达,钙化软骨层未见明显阳性表达,8周组与对照组相比有显著差异(P<0.05)。结论:关节盘前移位后早期髁突软骨VEGF-C基因的转录与表达有所下降,而在后期升高,可能参与关节盘移位后髁突软骨的改建。PURPOSE： To investigate the expression and significance of vascular endothelial growth factor-C in the condylar cartilage after anterior disc displacement of temporomandibular joint. METHODS： Sixty healthy adult Japanese rabbits were used in this study. Forty-eight rabbits were subjected to surgical anterior disc displacement in the right TMJs,every 12 rabbits were sacrificed 1, 2, 4, 8 weeks post-operation, 12 rabbits without operation were used as normal control.The gene expression and distribution of VEGF-C in condylar cartilage were studied by gene chip, real-time quantitative PCR and SP immunohistochemical methods. Paired t test was used for analysis with SPSS19.0 software package.RESULTS： After anterior disc displacement, the expression of VEGF-C in the 2nd week reached to a minimum level,there was a significant difference（P0.05）, and increased in the 8th week. VEGF-C was expressed by cells of articular cartilage in the proliferative zone, hypertrophic zone and calcified cartilage zone. The expression in 1-week group was mainly found in the deep proliferative zone and hypertrophic zone, while the expression mainly found in the hypertrophic zone in 2-and 4-week group; calcified cartilage gradually enhanced, and there was significant difference（ P0.05） between8-week group and control group. CONCLUSIONS： VEGF-C gene transcription and expression may be involved in theremodeling of the condylar cartilage after anterior disc displacement.

关 键 词：关节盘前移 髁突 VEGF-C 基因芯片 

分 类 号：R782.6[医药卫生—口腔医学]