中晚期肝癌围介入期血清CXCL9和HSP90水平的变化及其临床意义  

Changes of serum CXCL9 and HSP90 levels during interventional perioperative period in patients with middle-late stage liver cancer and their clinical significance

作　　者：钟胜[1] 蔡越飞[1] 谭小明[1] 王有枝[1] 冼重扬 简燕[1] 

机构地区：[1]中山大学附属东华医院介入科,广东东莞523110

出　　处：《介入放射学杂志》2016年第4期312-315,共4页Journal of Interventional Radiology

摘　　要：目的探讨中晚期肝癌围介入期血清趋化因子9(CXCL9)和热休克蛋白90(HSP90)水平的变化及其与患者预后情况的关系。方法选取2010年1月至2012年6月拟行介入治疗的中晚期肝癌患者68例为肝癌组,另选取30例同期健康体检志愿者为对照。采用酶联免疫吸附法(ELISA)检测对照组和肝癌组介入治疗前(T_0)、介入治疗后1 d(T_1)、3 d(T_2)、7 d(T_3)及10 d(T_4)的血清甲胎蛋白(AFP)、CXCL9和HSP90水平。采用Kaplan-Meier生存曲线进行肝癌组随访3年的生存分析并分别采用Spearman秩相关法分析中晚期肝癌围介入期血清CXCL9、HSP90、AFP水平和3年生存率的关系,采用Logistic多因素分析法分析中晚期肝癌围介入期血清CXCL9和HSP90水平对其预后的影响。结果肝癌组围介入期的血清AFP、CXCL9和HSP90水平均明显高于对照组;与T0比较,肝癌组T3和T4的血清AFP水平、CXCL9和HSP90水平均明显降低(P<0.05)。肝癌组随访3年的生存率为67.6%,且存活患者围介入期的血清CXCL9和HSP90水平均低于死亡患者(P<0.05)。Spearman秩相关法分析结果显示,中晚期肝癌围介入期血清CXCL9和HSP90水平与其血清AFP水平呈正相关(r=-0.728,-0.746,P<0.05),与其3年生存率则均呈负相关(r=-0.728,-0.792,P<0.05)。Logistic多因素分析结果显示,血清CXCL9和HSP90水平均为其预后情况的独立相关因素。结论中晚期肝癌围介入期血清CXCL9和HSP90水平较高且均与其预后情况密切相关,中晚期肝癌患者的围介入期血清CXCL9和HSP90可能作为其预后情况评估的参考指标。Objective To explore the changes of serum chemokine 9（CXCL9） and heat shock protein90（HSP90） levels during interventional perioperative period in patients with middle-late stage liver cancer and to discuss their relationships with the prognosis. Methods A total of 68 patients with middle-late stage liver cancer, who were admitted to authors＇ hospital during the period from January 2010 to June 2012 and planned to receive interventional treatment, were collected as the study group; other 30 healthy volunteers who underwent health screening during the same period were selected as the control group. Using enzyme-linked immunosorbent（ELISA） method, the serum alpha-fetoprotein（AFP）, CXCL9 and HSP90 levels of all subjects in both groups were determined before interventional therapy（T_0） and at 1 d（T_1）, 3 d（T_2）, 7 d（T_3）and 10 d（T_4） after interventional therapy. Kaplan Meier-survival curve was adopted to analyze the 3-year survival rate of the study group, and Spearman rank correlation method was used to analyze the relationship between the perioperative serum AFP, CXCL9 and HSP90 levels and the 3-year survival rate, besides,Logistic multiple factors analysis was employed to evaluate the correlation of perioperative serum CXCL9 and HSP90 levels with the patient＇s prognosis. Results In the study group the serum AFP, CXCL9 and HSP90 levels of T0 were evidently higher than those in the control group, and the serum AFP, CXCL9 and HSP90 levels of T3 and T4were remarkably lower than those of T0（P〈0.05）. The 3-year survival rate of the study group was 67.6%, and the perioperative serum CXCL9 and HSP90 levels of the survivors were significantly lower than those of the patients who died（P〈0.05）. Spearman rank correlation analysis revealed that in the study group a parallel correlation existed between the perioperative serum CXCL9 and HSP90 levels and the perioperative serum AFP levels（r=-0.728 and r=-0.746 respectively, P〈0.05）, while a negative correlation existed bet

关 键 词：中晚期 肝癌 介入治疗 CXCL9 HSP90 预后 生存率 

分 类 号：R735.7[医药卫生—肿瘤]