AT模体结合因子1对结直肠癌LOVO细胞增殖侵袭的影响  

Effect of AT Motif Binding Factor 1 on Proliferation and Invasion of Colorectal Carcinoma Cell Line LOVO

作　　者：林志群[1] 赵大川[1] 陈飞[1] 黄宗海[1] 杨少华[1] 赵海军[1] 莫林海[1] 

机构地区：[1]南方医科大学珠江医院普通外科,广州510280

出　　处：《肿瘤防治研究》2016年第4期267-271,共5页Cancer Research on Prevention and Treatment

摘　　要：目的构建ATBF1高表达的LOVO细胞,并观察其增殖性、凋亡率、侵袭性的变化。方法将p EGFP-ATBF1转染入LOVO细胞后,在荧光显微镜下观察其转染情况,通过RT-PCR、Western blot检测并研究转染后ATBF1、Notch3的表达情况。利用CCK-8(Cell Counting Kit-8)和小室迁移实验分别研究LOVO细胞的增殖状态与侵袭能力,通过流式细胞术探索ATBF1对LOVO凋亡的影响。结果与空白组(未处理组)、对照组(转染p EGFP质粒)对比,实验组(转染p EGFP-ATBF1质粒)LOVO细胞的增殖性、侵袭性明显受到抑制(P<0.001),流式细胞术提示实验组LOVO细胞的凋亡率为15.9%,空白组和对照组的凋亡率仅为5.2%和4.6%,而Western blot分析结果表明实验组癌基因Notch3表达量较其他两组有所下降(P<0.05)。结论抑癌基因ATBF1可抑制LOVO的增殖性及侵袭性,并促进其凋亡,同时可下调Notch3的表达,这意味着Notch3有可能是ATBF1的一个下游基因。Objective To establish the cell model of LOVO with overexpressed AT motif binding factor 1（ATBF1）, and to observe the change of the proliferation, apoptosis, invasion of LOVO cell line. Methods The result was detected under a fluorescent microscope after p EGFP-ATBF1 was transfected into human colorectal carcinoma cell line LOVO by Lipofectamine. The expression of ATBF1 and Notch3 were identified by RT-PCR and Western blot. CCK-8 method was used to measure cell growth and Transwell migration assay was utilized to detect the invasion of overexpressed ATBF1 LOVO. The relationship between ATBF1 and the apoptosis of LOVO cells was analyzed by flow cytometry. Results Compared with blank group and control group, the proliferation and invasion of overexprssed ATBF1 LOVO were inhibited in the trial group（P〈0.001）. There were higher apoptosis rate of LOVO cells（15.9%） in treatment group than those in blank（5.2%） and control groups（4.6%） according to the result of flow cytometry. However, Western blot showed that the expression of oncogene Notch3 descended in the trial group（P〈0.05）. Conclusion Anti-oncogene ATBF1 could inhibit the proliferation and invasion of LOVO cells and induce its apoptosis, meanwhile, ATBF1 could downregulate the expression of Notch3, which means Notch3 might be one of the downstream gene of ATBF1.

关 键 词：AT模体结合因子1 抑癌基因 增殖 侵袭 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤]